Detergents: Friends not foes for high‐performance membrane proteomics toward precision medicine
Sign Up to like & getrecommendations! Published in 2017 at "PROTEOMICS"
DOI: 10.1002/pmic.201600209
Abstract: Precision medicine, particularly therapeutics, emphasizes the atomic‐precise, dynamic, and systems visualization of human membrane proteins and their endogenous modifiers. For years, bottom‐up proteomics has grappled with removing and avoiding detergents, yet faltered at the therapeutic‐pivotal… read more here.
Keywords: medicine; precision medicine; membrane proteomics; toward precision ... See more keywords
Membrane Proteomics in Gram-Positive Bacteria: Two Complementary Approaches to Target the Hydrophobic Species of Proteins.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8695-8_3
Abstract: This protocol represents a detailed instruction how to prepare protein samples in order to raise mass spectrometry-based identification and quantification rates with respect to the challenging class of membrane proteins. This will increase comprehensiveness of… read more here.
Keywords: protein; gram positive; membrane proteins; proteins membrane ... See more keywords
Efficient Protein Digestion at Elevated Temperature in the Presence of SDS and Calcium Ions for Membrane Proteomics.
Sign Up to like & getrecommendations! Published in 2019 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.9b00484
Abstract: Growing significance of membrane proteins inspires continuous development and improvement of methods for robust membrane proteomics. Here, we developed a very simple and efficient method for membrane protein digestion using an ionic detergent SDS at… read more here.
Keywords: protein digestion; temperature; calcium ions; membrane proteomics ... See more keywords