Extra metabolic burden by displaying over secreting: Growth, fermentation and enzymatic activity in cellobiose of recombinant yeast expressing β-glucosidase.
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DOI: 10.1016/j.biortech.2017.12.030
Abstract: β-Glucosidase was selected to be a reporter to study metabolic burden imposed by its expression in yeast. Cell growth, fermentation yield and enzymatic activity were used as indicators of the metabolic burden borne by 14… read more here.
Keywords: yeast; metabolic burden; glucosidase; growth ... See more keywords
Proteomics and metabolic burden analysis to understand the impact of recombinant protein production in E. coli
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DOI: 10.1038/s41598-024-63148-y
Abstract: The impact of recombinant protein production (RPP) on host cells and the metabolic burden associated with it undermine the efficiency of the production system. This study utilized proteomics to investigate the dynamics of parent and… read more here.
Keywords: protein; metabolic burden; impact recombinant; protein production ... See more keywords
Ameliorating the Metabolic Burden of the Co-expression of Secreted Fungal Cellulases in a High Lipid-Accumulating Yarrowia lipolytica Strain by Medium C/N Ratio and a Chemical Chaperone
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DOI: 10.3389/fmicb.2018.03276
Abstract: Yarrowia lipolytica, known to accumulate lipids intracellularly, lacks the cellulolytic enzymes needed to break down solid biomass directly. This study aimed to evaluate the potential metabolic burden of expressing core cellulolytic enzymes in an engineered… read more here.
Keywords: high lipid; chemical chaperone; expression; metabolic burden ... See more keywords