13C‐metabolic flux analysis of human adenovirus infection: Implications for viral vector production
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26063
Abstract: Adenoviruses are human pathogens increasingly used as gene therapy and vaccination vectors. However, their impact on cell metabolism is poorly characterized. We performed carbon labeling experiments with [1,2‐13C]glucose or [U‐13C]glutamine to evaluate metabolic alterations in… read more here.
Keywords: metabolic flux; production; 13c metabolic; infection ... See more keywords
Effect of light fluctuations on photosynthesis and metabolic flux in Synechocystis sp. PCC 6803.
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology progress"
DOI: 10.1002/btpr.3326
Abstract: In nature, photosynthetic organisms are exposed to fluctuating light, and their physiological systems must adapt to this fluctuation. To maintain homeostasis, these organisms have a light fluctuation photoprotective mechanism, which functions in both photosystems and… read more here.
Keywords: pcc 6803; synechocystis pcc; light fluctuations; metabolic flux ... See more keywords
Input function and modeling for determining bone metabolic flux using [18 F] sodium fluoride PET imaging: a step-by-step guide.
Sign Up to like & getrecommendations! Published in 2022 at "Medical physics"
DOI: 10.1002/mp.16125
Abstract: Studies of skeletal metabolism using measurements of bone metabolic flux (Ki ) obtained with [18 F] sodium fluoride ([18 F]NaF) positron emission tomography (PET) scans have been used in clinical research for the last 30… read more here.
Keywords: bone; pet; input function; metabolic flux ... See more keywords
A new strategy for dynamic metabolic flux estimation by integrating transient metabolome data into genome-scale metabolic models
Sign Up to like & getrecommendations! Published in 2021 at "Bioprocess and Biosystems Engineering"
DOI: 10.1007/s00449-021-02626-3
Abstract: Metabolic flux analysis (MFA) is a powerful tool for studying microbial cell physiology. Isotope-based MFA is the accepted standard for studying metabolic fluxes under steady-state conditions. However, its application under dynamic extracellular conditions is limited… read more here.
Keywords: metabolic flux; data genome; genome scale; physiology ... See more keywords
Metabolic Flux Analysis of Simultaneous Production of Vitamin B12 and Propionic Acid in a Coupled Fermentation Process by Propionibacterium freudenreichii
Sign Up to like & getrecommendations! Published in 2021 at "Applied Biochemistry and Biotechnology"
DOI: 10.1007/s12010-021-03584-y
Abstract: The metabolic processes involved in simultaneous production of vitamin B12 and propionic acid by Propionibacterium freudenreichii are very complicated. To further investigate the regulatory mechanism of this metabolism, a simplified metabolic network was established. The… read more here.
Keywords: metabolic flux; propionic acid; vitamin b12; fermentation ... See more keywords
In Vivo Estimates of Liver Metabolic Flux Assessed by 13C-Propionate and 13C-Lactate Are Impacted by Tracer Recycling and Equilibrium Assumptions.
Sign Up to like & getrecommendations! Published in 2020 at "Cell reports"
DOI: 10.1016/j.celrep.2020.107986
Abstract: Isotope-based assessment of metabolic flux is achieved through a judicious balance of measurements and assumptions. Recent publications debate the validity of key assumptions used to model stable isotope labeling of liver metabolism in vivo. Here, we… read more here.
Keywords: metabolic flux; estimates liver; lactate; propionate ... See more keywords
Biological insights into non-model microbial hosts through stable-isotope metabolic flux analysis.
Sign Up to like & getrecommendations! Published in 2019 at "Current opinion in biotechnology"
DOI: 10.1016/j.copbio.2019.09.010
Abstract: Stable-isotope metabolic flux analysis is an important approach to unravel the metabolic network and its regulation in organisms. It has become a key analytical technology for biotechnological applications. During recent years non-model microorganisms have received… read more here.
Keywords: metabolic flux; stable isotope; flux analysis; isotope metabolic ... See more keywords
Combined metabolomic and transcriptomic analysis reveals redirection of the phenylpropanoid metabolic flux in different colored medicinal Chrysanthemum morifolium
Sign Up to like & getrecommendations! Published in 2021 at "Industrial Crops and Products"
DOI: 10.1016/j.indcrop.2021.113343
Abstract: Abstract As a medicinal and edible plant, Chrysanthemum morifolium cv. "Hangju" has economic value and is widely used as a Chinese herbal medicine and tea. However, the metabolomic difference between "Hangju" flowers with white and… read more here.
Keywords: colored medicinal; metabolic flux; chrysanthemum morifolium; different colored ... See more keywords
13C metabolic flux analysis of three divergent extremely thermophilic bacteria: Geobacillus sp. LC300, Thermus thermophilus HB8, and Rhodothermus marinus DSM 4252.
Sign Up to like & getrecommendations! Published in 2017 at "Metabolic engineering"
DOI: 10.1016/j.ymben.2017.10.007
Abstract: Thermophilic organisms are being increasingly investigated and applied in metabolic engineering and biotechnology. The distinct metabolic and physiological characteristics of thermophiles, including broad substrate range and high uptake rates, coupled with recent advances in genetic… read more here.
Keywords: metabolic flux; engineering; 13c metabolic; three divergent ... See more keywords
Ambient Ultraviolet B Signal Modulates Tea Flavor Characteristics via Shifting a Metabolic Flux in Flavonoid Biosynthesis.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.0c07009
Abstract: Tea leaves contain an extraordinarily high level of flavonoids that contribute to tea health benefits and flavor characteristics, but the regulatory mechanism of ambient ultraviolet B (UV-B) on tea flavonoid enrichment remains unclear. Here, we… read more here.
Keywords: metabolic flux; flux flavonoid; modulates tea; ambient ultraviolet ... See more keywords
Metabolic Flux Enhancement and Transcriptomic Analysis Displayed the Changes of Catechins Following Long-Term Pruning in Tea Trees ( Camellia sinensis).
Sign Up to like & getrecommendations! Published in 2018 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.8b02877
Abstract: The tea tree is a perennial woody plant, and pruning is one of the most crucial cultivation measurements for tea plantation management. To date, the relationship between long-term pruning and metabolic flux enhancement in tea… read more here.
Keywords: metabolic flux; long term; analysis; tea trees ... See more keywords