Metal-Organic-Framework-Assisted In Vivo Bacterial Metabolic Labeling and Precise Antibacterial Therapy.
Sign Up to like & getrecommendations! Published in 2018 at "Advanced materials"
DOI: 10.1002/adma.201706831
Abstract: Bacterial infection is one of the most serious physiological conditions threatening human health. There is an increasing demand for more effective bacterial diagnosis and treatment through noninvasive theranostic approaches. Herein, a new strategy is reported… read more here.
Keywords: framework assisted; metal organic; metabolic labeling; therapy ... See more keywords
Metabolic Labeling of Inositol Phosphates and Phosphatidylinositols in Yeast and Mammalian Cells.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0167-9_7
Abstract: Inositol phosphate (IP) and phosphatidylinositol (PI) signaling are critical signal transduction pathways responsible for generating numerous receptor-mediated cellular responses. Biochemical and genetic studies have revealed diverse roles of IP and PI signaling in eukaryotic signaling,… read more here.
Keywords: inositol; phosphates phosphatidylinositols; labeling inositol; inositol phosphates ... See more keywords
Metabolic Labeling of Clostridioides difficile Proteins.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1024-4_19
Abstract: The introduction of stable isotopes in vivo via metabolic labeling approaches (SILAC or 15N-labeling) allows, after combination of differentially treated labeled and unlabeled cells or protein extracts, for correction of protein quantification errors implemented during… read more here.
Keywords: clostridioides difficile; labeling clostridioides; biology; metabolic labeling ... See more keywords
Metabolic Labeling of Surface Neo-sialylglyconjugates Catalyzed by Trypanosoma cruzi trans-Sialidase.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9148-8_10
Abstract: Trypanosoma cruzi, the protozoan agent of Chagas disease, has evolved an innovative metabolic pathway by which protective sialic acid (SA) residues are scavenged from host sialylglycoconjugates and transferred onto parasite surface mucin-like molecules (or surface… read more here.
Keywords: trans sialidase; surface; cruzi; metabolic labeling ... See more keywords
Metabolic Labeling of Glycerophospholipids via Clickable Analogs Derivatized at the Lipid Headgroup.
Sign Up to like & getrecommendations! Published in 2020 at "Chemistry and physics of lipids"
DOI: 10.1016/j.chemphyslip.2020.104971
Abstract: Metabolic labeling, in which substrate analogs containing diminutive tags can infiltrate biosynthetic pathways and generate labeled products in cells, has led to dramatic advancements in the means by which complex biomolecules can be detected and… read more here.
Keywords: clickable analogs; glycerophospholipids via; headgroup; labeling glycerophospholipids ... See more keywords
Proteome Dynamics from Heavy Water Metabolic Labeling and Peptide Tandem Mass Spectrometry.
Sign Up to like & getrecommendations! Published in 2019 at "International journal of mass spectrometry"
DOI: 10.1016/j.ijms.2019.116194
Abstract: Protein homeostasis (proteostasis) is a result of a dynamic equilibrium between protein synthesis and degradation. It is important for healthy cell/organ functioning and is often associated with diseases such as neurodegenerative diseases and non-Alcoholic Fatty… read more here.
Keywords: heavy water; metabolic labeling; mass spectrometry; mass ... See more keywords
An Optimized Enzyme-Nucleobase Pair Enables In Vivo RNA Metabolic Labeling with Improved Cell-Specificity.
Sign Up to like & getrecommendations! Published in 2022 at "Biochemistry"
DOI: 10.1021/acs.biochem.2c00559
Abstract: Current transcriptome-wide analyses have identified a growing number of regulatory RNA with expression that is characterized in a cell-type-specific manner. Herein, we describe RNA metabolic labeling with improved cell-specificity utilizing the in vivo expression of… read more here.
Keywords: cell; cell specificity; labeling improved; metabolic labeling ... See more keywords
trans-Cyclooctene- and Bicyclononyne-Linked Nucleotides for Click Modification of DNA with Fluorogenic Tetrazines and Live Cell Metabolic Labeling and Imaging
Sign Up to like & getrecommendations! Published in 2023 at "Bioconjugate Chemistry"
DOI: 10.1021/acs.bioconjchem.3c00064
Abstract: A series of 2′-deoxyribonucleoside triphosphates (dNTPs) bearing 2- or 4-linked trans-cyclooctene (TCO) or bicyclononyne (BCN) tethered through a shorter propargylcarbamate or longer triethyleneglycol-based spacer were designed and synthesized. They were found to be good substrates… read more here.
Keywords: trans cyclooctene; dna; bicyclononyne; metabolic labeling ... See more keywords
Using Heavy Mass Isotopomers for Protein Turnover in Heavy Water Metabolic Labeling.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.0c00873
Abstract: Metabolic labeling followed by LC-MS-based proteomics is a powerful tool to study proteome dynamics in high-throughput experiments both in vivo and in vitro. High mass resolution and accuracy allow differentiation in isotope profiles and the… read more here.
Keywords: mass; heavy mass; heavy water; metabolic labeling ... See more keywords
TurnoveR: A Skyline External Tool for Analysis of Protein Turnover in Metabolic Labeling Studies.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.2c00173
Abstract: In spite of its central role in biology and disease, protein turnover is a largely understudied aspect of most proteomic studies due to the complexity of computational workflows that analyze in vivo turnover rates. To… read more here.
Keywords: metabolic labeling; turnover rates; analysis; protein turnover ... See more keywords
Metabolic Labeling Strategy Boosted Antibacterial Efficiency for Photothermal and Photodynamic Synergistic Bacteria-Infected Wound Therapy.
Sign Up to like & getrecommendations! Published in 2022 at "ACS applied materials & interfaces"
DOI: 10.1021/acsami.2c15759
Abstract: Pathogenic bacteria infections bring about a substantial risk to human health. Given the development of antibiotic-resistance bacteria, alternative antibacterial strategies with great inactivation efficiency and bacteria-binding ability are extremely attractive. In this work, a metabolic… read more here.
Keywords: photothermal photodynamic; antibacterial efficiency; daa; metabolic labeling ... See more keywords