In vivo calibration of genetically-encoded metabolite biosensors must account for metabolite metabolism during calibration and cellular volume.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of neurochemistry"
DOI: 10.1111/jnc.15775
Abstract: Isotopic assays of brain glucose utilization rates have been used for more than four decades to establish relationships between energetics, functional activity, and neurotransmitter cycling. Limitations of these methods include the relatively long time (1-60… read more here.
Keywords: metabolism; calibration; lactate; genetically encoded ... See more keywords
Surveying the Genetic Design Space for Transcription Factor-Based Metabolite Biosensors: Synthetic Gamma-Aminobutyric Acid and Propionate Biosensors in E. coli Nissle 1917
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2022.938056
Abstract: Engineered probiotic bacteria have been proposed as a next-generation strategy for noninvasively detecting biomarkers in the gastrointestinal tract and interrogating the gut-brain axis. A major challenge impeding the implementation of this strategy has been the… read more here.
Keywords: design; factor based; transcription factor; metabolite biosensors ... See more keywords