Comparison of global metabolite extraction strategies for soybeans using UHPLC-HRMS
Sign Up to like & getrecommendations! Published in 2017 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-017-0557-6
Abstract: AbstractMetabolism, downstream effectors of genomics, transcriptomics, and proteomics, can determine the potential of phenotype of an organism including plants. Profiling the global scenario of metabolism requires optimization of different solvent extraction methods. Here, we report… read more here.
Keywords: uhplc hrms; metabolite extraction; extraction; extraction methods ... See more keywords
Metabolomic study of disease progression in scrapie prion infected mice; validation of a novel method for brain metabolite extraction
Sign Up to like & getrecommendations! Published in 2020 at "Metabolomics"
DOI: 10.1007/s11306-020-01690-2
Abstract: Introduction Prion disease is a form of neurodegenerative disease caused by the misfolding and aggregation of cellular prion protein (PrP C ). The neurotoxicity of the misfolded form of prion protein, PrP Sc still remains… read more here.
Keywords: metabolite extraction; prion; disease; spectroscopy ... See more keywords
Intracellular metabolite profiling and the evaluation of metabolite extraction solvents for Clostridium carboxidivorans fermenting carbon monoxide
Sign Up to like & getrecommendations! Published in 2020 at "Process Biochemistry"
DOI: 10.1016/j.procbio.2019.10.012
Abstract: Abstract Clostridium carboxidivorans ferments CO, CO2, and H2 via the Wood-Ljungdahl pathway. CO, CO2, and H2 are unique substrates, unlike other carbon sources like glucose, so it is necessary to analyze intracellular metabolite profiles for… read more here.
Keywords: metabolite extraction; extraction; clostridium carboxidivorans; intracellular metabolite ... See more keywords
SiMeEx, a simplified method for metabolite extraction of adherent mammalian cells
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Molecular Biosciences"
DOI: 10.3389/fmolb.2022.1084060
Abstract: A reliable method for metabolite extraction is central to mass spectrometry-based metabolomics. However, existing methods are lengthy, mostly due to the step of scraping cells from cell culture vessels, which restricts metabolomics in broader application… read more here.
Keywords: adherent mammalian; method metabolite; extraction; mammalian cells ... See more keywords