Identification and Characterization of a Novel Carboxylesterase Belonging to Family VIII with Promiscuous Acyltransferase Activity Toward Cyanidin-3-O-Glucoside from a Soil Metagenomic Library
Sign Up to like & getrecommendations! Published in 2021 at "Applied Biochemistry and Biotechnology"
DOI: 10.1007/s12010-021-03614-9
Abstract: An alkaline esterase, designated as EstXT1, was identified through functional screening from a metagenomic library. Sequence analysis revealed that EstXT1 belonged to the family VIII carboxylesterases and contained a characteristic conserved S-x-x-K motif and a… read more here.
Keywords: acyltransferase activity; metagenomic library; family viii; activity ... See more keywords
Unraveling the microbial and functional diversity of Coamo thermal spring in Puerto Rico using metagenomic library generation and shotgun sequencing
Sign Up to like & getrecommendations! Published in 2017 at "Genomics Data"
DOI: 10.1016/j.gdata.2016.12.010
Abstract: In Puerto Rico, the microbial diversity of the thermal spring (ThS) in Coamo has never been studied using metagenomics. The focus of our research was to generate a metagenomic library from the ThS of Coamo,… read more here.
Keywords: microbial functional; metagenomic library; functional diversity; puerto rico ... See more keywords
Characterization of truncated endo-β-1,4-glucanases from a compost metagenomic library and their saccharification potentials.
Sign Up to like & getrecommendations! Published in 2018 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2018.04.102
Abstract: A gene encoding an endo-β-1,4-glucanase (Cel6H-f481) was cloned from a compost metagenomic library. The gene, cel6H-f481, was composed of 1446 bp to encode a fused protein of 481 amino acid residues (50,429 Da), i.e., 445 residues (Cel6H-445)… read more here.
Keywords: compost metagenomic; metagenomic library; saccharification; cel6h ... See more keywords
A chlorogenic acid esterase from a metagenomic library with unique substrate specificity and its application in caffeic and ferulic acid production from agricultural byproducts
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DOI: 10.1080/10242422.2021.1969370
Abstract: Soil microbes are an abundant source of enzymes with unique properties that may be useful for industrial applications. As most wild-type strains show low chlorogenic acid esterase expression and ac... read more here.
Keywords: metagenomic library; library unique; esterase metagenomic; acid esterase ... See more keywords
Characterization of a Novel, Cold-Adapted, and Thermostable Laccase-Like Enzyme With High Tolerance for Organic Solvents and Salt and Potent Dye Decolorization Ability, Derived From a Marine Metagenomic Library
Sign Up to like & getrecommendations! Published in 2018 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2018.02998
Abstract: Synthetic dyes are widely used in many industries, but they cause serious environmental problems due to their carcinogenic and mutagenic properties. In contrast to traditional physical and chemical treatments, biodegradation is generally considered an environmental-friendly,… read more here.
Keywords: like enzyme; organic solvents; laccase like; metagenomic library ... See more keywords
Characterization of a novel thermostable alkaline lipase derived from a compost metagenomic library and its potential application in the detergent industry
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2022.1088581
Abstract: Using composted soil samples, a metagenomic library consisting of 36,000 clones was constructed. Then, a novel lipase, Lip54q, which belongs to the VIII family of lipolytic enzymes, was identified from the metagenomic library by functional… read more here.
Keywords: application; detergent industry; enzyme; substrate ... See more keywords
Characterization of a novel β-agarase from Antarctic macroalgae-associated bacteria metagenomic library and anti-inflammatory activity of the enzymatic hydrolysates
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2022.972272
Abstract: An agarase gene (aga1904) that codes a protein with 640 amino acids was obtained from the metagenomic library of macroalgae-associated bacteria collected from King George Island, Antarctica. Gene aga1904 was expressed in Escherichia coli BL21… read more here.
Keywords: macroalgae associated; associated bacteria; enzymatic hydrolysates; inflammatory ... See more keywords