Clinical application of metagenomic next‐generation sequencing technology in the diagnosis and treatment of pulmonary infection pathogens: A prospective single‐center study of 138 patients
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Clinical Laboratory Analysis"
DOI: 10.1002/jcla.24498
Abstract: Rapid and accurate pathogen identification is essential for the treatment of pneumonia. Metagenomic next‐generation sequencing (mNGS) is a newly developed technology to obtain microbial nucleic acid sequence information quickly, efficiently, and without bias. read more here.
Keywords: technology; next generation; metagenomic next; treatment ... See more keywords
Pathogen determination from clinical abscess fluids using metagenomic next-generation sequencing.
Sign Up to like & getrecommendations! Published in 2020 at "Folia microbiologica"
DOI: 10.1007/s12223-020-00829-x
Abstract: Abscesses are often clinically manifested as local necrotic tissues in various organs or systems of the human body, which is commonly caused by microbial infection. Rapid and accurate identification of pathogens from clinical abscetic samples… read more here.
Keywords: metagenomic next; culture; next generation; generation sequencing ... See more keywords
Early Identification of Fungal and Mycobacterium Infections in Pulmonary Granulomas Using Metagenomic Next-Generation Sequencing on Formalin fixation and paraffin embedding tissue
Sign Up to like & getrecommendations! Published in 2022 at "Expert Review of Molecular Diagnostics"
DOI: 10.1080/14737159.2022.2052046
Abstract: ABSTRACT Background The purpose of the study is to assess the etiology detection ability of Metagenomic Next-Generation Sequencing (mNGS) on formalin fixation and paraffin embedding (FFPE) tissue from postoperative biopsy specimens. Methods We prospectively enrolled… read more here.
Keywords: etiology; detection; next generation; metagenomic next ... See more keywords
The clinical application of metagenomic next-generation sequencing for detecting pathogens in bronchoalveolar lavage fluid: case reports and literature review
Sign Up to like & getrecommendations! Published in 2022 at "Expert Review of Molecular Diagnostics"
DOI: 10.1080/14737159.2022.2071607
Abstract: ABSTRACT Introduction Clinical metagenomic next-generation sequencing (mNGS) allows a comprehensive genetic analysis of microbial materials. Different from other traditional target-driven molecular diagnostic tests, such as PCR, mNGS is a hypothesis-free diagnostic approach that allows a… read more here.
Keywords: generation sequencing; next generation; clinical application; metagenomic next ... See more keywords
Application of metagenomic next-generation sequencing in the diagnosis and resistome analysis of community-acquired pneumonia pathogens from bronchoalveolar lavage samples.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of applied microbiology"
DOI: 10.1093/jambio/lxad102
Abstract: AIMS To perform a prospective diagnostic study exploring the clinical utility of metagenomic next-generation sequencing (mNGS) in diagnosing community-acquired pneumonia (CAP), and revealing resistome differences in bronchoalveolar lavage fluid (BALF) from CAP patients with varying… read more here.
Keywords: cap patients; cap; group; next generation ... See more keywords
Multisite Pseudomonas aeruginosa Infections Detected by Metagenomic Next-Generation Sequencing in a Child with Aplastic Anemia: A Case Report.
Sign Up to like & getrecommendations! Published in 2022 at "Laboratory medicine"
DOI: 10.1093/labmed/lmab123
Abstract: Microbial cultivation is the current gold standard for the clinical diagnosis of bacterial infections. However, this method sometimes produces false negative results. We present a case study of multisite Pseudomonas aeruginosa infections detected by metagenomic… read more here.
Keywords: aeruginosa infections; pseudomonas aeruginosa; infections detected; detected metagenomic ... See more keywords
Metagenomic next-generation sequencing for the identification of infections caused by Gram-negative pathogens and the prediction of antimicrobial resistance.
Sign Up to like & getrecommendations! Published in 2023 at "Laboratory medicine"
DOI: 10.1093/labmed/lmad039
Abstract: OBJECTIVE The aim of this study was to evaluate the efficacy of metagenomic next-generation sequencing (mNGS) for the identification of Gram-negative bacteria (GNB) infections and the prediction of antimicrobial resistance. METHODS A retrospective analysis was… read more here.
Keywords: resistance; next generation; metagenomic next; antimicrobial resistance ... See more keywords
The value of metagenomic next-generation sequencing for the diagnosis of pulmonary tuberculous using bronchoalveolar lavage fluid.
Sign Up to like & getrecommendations! Published in 2023 at "Laboratory medicine"
DOI: 10.1093/labmed/lmad041
Abstract: OBJECTIVE The aim of this study was to compare metagenomic next-generation sequencing (mNGS) with other methods, including Xpert MTB/RIF, Mycobacterium tuberculosis (MTB) culture, and acid-fast bacillus (AFB) staining in the diagnosis of pulmonary tuberculosis (PTB)… read more here.
Keywords: culture; mtb culture; diagnosis; next generation ... See more keywords
Metagenomic Next-Generation Sequencing for Pathogen Detection and Transcriptomic Analysis in Pediatric Central Nervous System Infections
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DOI: 10.1093/ofid/ofab104
Abstract: Abstract Background Pediatric central nervous system (CNS) infections are potentially life-threatening and may incur significant morbidity. Identifying a pathogen is important, both in terms of guiding therapeutic management and in characterizing prognosis. Usual care testing… read more here.
Keywords: metagenomic next; nervous system; central nervous; transcriptomic analysis ... See more keywords
2563. Clinical Metagenomic Next-Generation Sequencing for Diagnosis of Meningitis and Encephalitis
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DOI: 10.1093/ofid/ofy209.171
Abstract: Abstract Background Metagenomic next-generation sequencing (mNGS) of CSF can identify nearly all pathogens in a single test. We previously validated a CSF mNGS assay in a licensed clinical laboratory. To date, the utility of mNGS… read more here.
Keywords: diagnosis; metagenomic next; clinical metagenomic; encephalitis ... See more keywords
Metagenomic next-generation sequencing for the diagnosis of Pneumocystis jirovecii pneumonia in solid organ transplant recipients
Sign Up to like & getrecommendations! Published in 2022 at "Chinese Medical Journal"
DOI: 10.1097/cm9.0000000000002120
Abstract: To the Editor: Pneumocystis jirovecii (P. jirovecii) pneumonia (PJP) is an opportunistic infection that usually occurs in immunosuppressed patients. Solid organ transplant (SOT) recipients have a high incidence of PJP due to the use of… read more here.
Keywords: pneumocystis jirovecii; metagenomic next; jirovecii; organ transplant ... See more keywords