Diversity of DNA and RNA Viruses in Indoor Air As Assessed via Metagenomic Sequencing.
Sign Up to like & getrecommendations! Published in 2018 at "Environmental science & technology"
DOI: 10.1021/acs.est.7b04203
Abstract: Diverse bacterial and fungal communities inhabit human-occupied buildings and circulate in indoor air; however, viral diversity in these man-made environments remains largely unknown. Here we investigated DNA and RNA viruses circulating in the air of… read more here.
Keywords: rna viruses; diversity; metagenomic sequencing; air ... See more keywords
Deep insights into the gut microbial community of extreme longevity in south Chinese centenarians by ultra-deep metagenomics and large-scale culturomics
Sign Up to like & getrecommendations! Published in 2022 at "npj Biofilms and Microbiomes"
DOI: 10.1038/s41522-022-00282-3
Abstract: The gut microbes play important roles in human longevity and the gut microbiota profile of centenarians shows some unique features from young adults. Nowadays, most microbial studies on longevity are commonly based on metagenomic sequencing… read more here.
Keywords: metagenomic sequencing; deep insights; large scale; longevity ... See more keywords
Metagenomic sequencing with spiked primer enrichment for viral diagnostics and genomic surveillance
Sign Up to like & getrecommendations! Published in 2020 at "Nature Microbiology"
DOI: 10.1038/s41564-019-0637-9
Abstract: Metagenomic next-generation sequencing (mNGS), the shotgun sequencing of RNA and DNA from clinical samples, has proved useful for broad-spectrum pathogen detection and the genomic surveillance of viral outbreaks. An additional target enrichment step is generally… read more here.
Keywords: sequencing spiked; detection; spiked primer; metagenomic sequencing ... See more keywords
Culture-enriched metagenomic sequencing enables in-depth profiling of the cystic fibrosis lung microbiota
Sign Up to like & getrecommendations! Published in 2020 at "Nature Microbiology"
DOI: 10.1038/s41564-019-0643-y
Abstract: Amplicon sequencing (for example, of the 16S rRNA gene) identifies the presence and relative abundance of microbial community members. However, metagenomic sequencing is needed to identify the genetic content and functional potential of a community.… read more here.
Keywords: cystic fibrosis; metagenomic sequencing; culture enriched; fibrosis lung ... See more keywords
A streamlined clinical metagenomic sequencing protocol for rapid pathogen identification.
Sign Up to like & getrecommendations! Published in 2021 at "Scientific reports"
DOI: 10.1038/s41598-021-83812-x
Abstract: Metagenomic next-generation sequencing (mNGS) holds promise as a diagnostic tool for unbiased pathogen identification and precision medicine. However, its medical utility depends largely on assay simplicity and reproducibility. In the current study, we aimed to… read more here.
Keywords: identification; metagenomic sequencing; pathogen identification; protocol rapid ... See more keywords
Optimization of cerebrospinal fluid microbial DNA metagenomic sequencing diagnostics
Sign Up to like & getrecommendations! Published in 2022 at "Scientific Reports"
DOI: 10.1038/s41598-022-07260-x
Abstract: Infection in the central nervous system is a severe condition associated with high morbidity and mortality. Despite ample testing, the majority of encephalitis and meningitis cases remain undiagnosed. Metagenomic sequencing of cerebrospinal fluid has emerged… read more here.
Keywords: pathogen; cerebrospinal fluid; metagenomic sequencing; dna metagenomic ... See more keywords
Targeted metagenomic sequencing data of human gut microbiota associated with Blastocystis colonization
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Data"
DOI: 10.1038/sdata.2017.81
Abstract: In the past decade, metagenomics studies have become widespread due to the arrival of second-generation sequencing platforms characterized by low costs, high throughput and short read lengths. Today, although benchtop sequencers are considered to be… read more here.
Keywords: targeted metagenomic; metagenomic sequencing; gut microbiota; microbiota ... See more keywords
Viral metagenomic sequencing in the diagnosis of meningoencephalitis: a review of technical advances and diagnostic yield.
Sign Up to like & getrecommendations! Published in 2021 at "Expert review of molecular diagnostics"
DOI: 10.1080/14737159.2021.1985467
Abstract: INTRODUCTION Meningoencephalitis patients are often severely impaired and benefit from early etiological diagnosis though many cases remain without identified cause. Metagenomics as pathogen agnostic approach can result in additional etiological findings, however the exact diagnostic… read more here.
Keywords: metagenomic sequencing; sequencing diagnosis; diagnostic yield; meningoencephalitis ... See more keywords
Managing the Transition to Widespread Metagenomic Monitoring: Policy Considerations for Future Biosurveillance
Sign Up to like & getrecommendations! Published in 2023 at "Health Security"
DOI: 10.1089/hs.2022.0029
Abstract: The technological possibilities and future public health importance of metagenomic sequencing have received extensive attention, but there has been little discussion about the policy and regulatory issues that need to be addressed if metagenomic sequencing… read more here.
Keywords: biosurveillance; policy; monitoring; metagenomic sequencing ... See more keywords
An informative approach on differential abundance analysis for time‐course metagenomic sequencing data
Sign Up to like & getrecommendations! Published in 2017 at "Bioinformatics"
DOI: 10.1093/bioinformatics/btw828
Abstract: Motivation: The advent of high‐throughput next generation sequencing technology has greatly promoted the field of metagenomics where previously unattainable information about microbial communities can be discovered. Detecting differentially abundant features (e.g. species or genes) plays… read more here.
Keywords: sequencing data; abundant features; metagenomic sequencing; time ... See more keywords
Real-time Utilization of Metagenomic Sequencing in the Diagnosis and Treatment Monitoring of an Invasive Adenovirus B55 Infection and Subsequent Herpes Simplex Virus Encephalitis in an Immunocompetent Young Adult
Sign Up to like & getrecommendations! Published in 2018 at "Open Forum Infectious Diseases"
DOI: 10.1093/ofid/ofy114
Abstract: Abstract Background Metagenomic sequencing has shown tremendous promise in solving difficult infectious diseases cases. In this study, we utilized this technology to help guide the care of a critically ill patient with severe pneumonia, fever… read more here.
Keywords: metagenomic sequencing; treatment; encephalitis; b55 ... See more keywords