Improved method for the extraction of high-quality DNA from lignocellulosic compost samples for metagenomic studies.
Sign Up to like & getrecommendations! Published in 2021 at "Applied microbiology and biotechnology"
DOI: 10.1007/s00253-021-11647-7
Abstract: The world economy is currently moving towards more sustainable approaches. Lignocellulosic biomass has been widely used as a substitute for fossil sources since it is considered a low-cost bio-renewable resource due to its abundance and… read more here.
Keywords: dna; high quality; compost samples; compost ... See more keywords
Metagenomic Studies in Inflammatory Skin Diseases
Sign Up to like & getrecommendations! Published in 2020 at "Current Microbiology"
DOI: 10.1007/s00284-020-02163-4
Abstract: Next-generation sequencing (NGS) technologies together with an improved access to compute performance led to a cost-effective genome sequencing over the past several years. This allowed researchers to fully unleash the potential of genomic and metagenomic… read more here.
Keywords: studies inflammatory; inflammatory skin; microbiology; metagenomic studies ... See more keywords
MetaBMF: a scalable binning algorithm for large-scale reference-free metagenomic studies
Sign Up to like & getrecommendations! Published in 2020 at "Bioinformatics"
DOI: 10.1093/bioinformatics/btz577
Abstract: MOTIVATION Metagenomics studies microbial genomes in an ecosystem such as the gastrointestinal tract of a human. Identification of novel microbial species and quantification of their distributional variations among different samples that are sequenced using next-generation-sequencing… read more here.
Keywords: metabmf scalable; different samples; matrix; binning ... See more keywords
Assessment of fecal DNA extraction protocols for metagenomic studies
Sign Up to like & getrecommendations! Published in 2020 at "GigaScience"
DOI: 10.1093/gigascience/giaa071
Abstract: Abstract Background Shotgun metagenomic sequencing has improved our understanding of the human gut microbiota. Various DNA extraction methods have been compared to find protocols that robustly and most accurately reflect the original microbial community structures.… read more here.
Keywords: extraction; dna; extraction protocols; protocol ... See more keywords
Contamination Issue in Viral Metagenomics: Problems, Solutions, and Clinical Perspectives
Sign Up to like & getrecommendations! Published in 2021 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2021.745076
Abstract: We describe the most common internal and external sources and types of contamination encountered in viral metagenomic studies and discuss their negative impact on sequencing results, particularly for low-biomass samples and clinical applications. We also… read more here.
Keywords: contamination issue; issue viral; contamination; metagenomics problems ... See more keywords
Metagenomic Studies of Viruses in Weeds and Wild Plants: A Powerful Approach to Characterise Variable Virus Communities
Sign Up to like & getrecommendations! Published in 2021 at "Viruses"
DOI: 10.3390/v13101939
Abstract: High throughput sequencing (HTS) has revolutionised virus detection and discovery, allowing for the untargeted characterisation of whole viromes. Viral metagenomics studies have demonstrated the ubiquity of virus infection – often in the absence of disease… read more here.
Keywords: characterise; weeds wild; studies viruses; metagenomic studies ... See more keywords