Defining the metal specificity of a multifunctional biofilm adhesion protein
Sign Up to like & getrecommendations! Published in 2017 at "Protein Science"
DOI: 10.1002/pro.3232
Abstract: The accumulation associated protein (Aap) of Staphylococcus epidermidis mediates intercellular adhesion events necessary for biofilm growth. This process depends upon Zn2+‐induced self‐assembly of G5 domains within the B‐repeat region of the protein, forming anti‐parallel, intertwined… read more here.
Keywords: protein; adhesion; metal specificity; defining metal ... See more keywords
Copy number of ArsR reporter plasmid determines its arsenite response and metal specificity
Sign Up to like & getrecommendations! Published in 2018 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-018-9042-1
Abstract: The key component in bacteria-based biosensors is a transcriptional reporter employed to monitor induction or repression of a reporter gene corresponding to environmental change. In this study, we made a series of reporters in order… read more here.
Keywords: metal specificity; copy number; reporter; copy ... See more keywords
An evolutionary path to altered cofactor specificity in a metalloenzyme
Sign Up to like & getrecommendations! Published in 2020 at "Nature Communications"
DOI: 10.1038/s41467-020-16478-0
Abstract: Almost half of all enzymes utilize a metal cofactor. However, the features that dictate the metal utilized by metalloenzymes are poorly understood, limiting our ability to manipulate these enzymes for industrial and health-associated applications. The… read more here.
Keywords: cofactor specificity; evolutionary path; metal; metal specificity ... See more keywords
The protein scaffold calibrates metal specificity and activation in MerR sensors
Sign Up to like & getrecommendations! Published in 2022 at "Microbial Biotechnology"
DOI: 10.1111/1751-7915.14151
Abstract: MerR metalloregulators are the central components of many biosensor platforms designed to report metal contamination. However, most MerR proteins are non‐specific. This makes it difficult to apply these biosensors in the analysis of real environmental… read more here.
Keywords: calibrates metal; scaffold calibrates; metal specificity; protein scaffold ... See more keywords