Journey of oocyte from metaphase‐I to metaphase‐II stage in mammals
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Cellular Physiology"
DOI: 10.1002/jcp.26467
Abstract: In mammals, journey from metaphase‐I (M‐I) to metaphase‐II (M‐II) is important since oocyte extrude first polar body (PB‐I) and gets converted into haploid gamete. The molecular and cellular changes associated with meiotic cell cycle progression… read more here.
Keywords: oocyte metaphase; metaphase metaphase; metaphase; journey oocyte ... See more keywords
Animal Lymphocyte Metaphase Chromosome Preparation.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9432-8_2
Abstract: Metaphase chromosome analysis of lymphocytes is the gold standard for biodosimetry to estimate the levels of radiation exposure in various animals as well as humans. Animals, including experimental, companion, and wild animals, are powerful and… read more here.
Keywords: metaphase chromosome; chromosome preparation; metaphase; animal lymphocyte ... See more keywords
Electron tomography reveals aspects of spindle structure important for mechanical stability at metaphase
Sign Up to like & getrecommendations! Published in 2019 at "Molecular Biology of the Cell"
DOI: 10.1091/mbc.e19-07-0405
Abstract: Metaphase spindles exert pole-directed forces on still-connected sister kinetochores. The spindle must counter these forces with extensive forces to prevent spindle collapse. In small spindles, kinetochore microtubules (KMTs) connect directly with the poles, and countering… read more here.
Keywords: metaphase; structure; metaphase spindles; electron tomography ... See more keywords
Helical coiling of metaphase chromatids
Sign Up to like & getrecommendations! Published in 2023 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkad028
Abstract: Abstract Chromatids of mitotic chromosomes were suggested to coil into a helix in early cytological studies and this assumption was recently supported by chromosome conformation capture (3C) sequencing. Still, direct differential visualization of a condensed… read more here.
Keywords: coiling metaphase; metaphase; helical coiling; metaphase chromatids ... See more keywords
The restriction of calcium influx in metaphase and post‐metaphase stages of cell division revealed by imaging secondary ion mass spectrometry (SIMS)
Sign Up to like & getrecommendations! Published in 2023 at "Journal of Microscopy"
DOI: 10.1111/jmi.13182
Abstract: A secondary ion mass spectrometry (SIMS)‐based isotopic imaging technique of ion microscopy was used for observing calcium influx in single renal epithelial LLC‐PK1 cells. The CAMECA IMS‐3f SIMS instrument, used in the study, is capable… read more here.
Keywords: metaphase; calcium; restriction; calcium influx ... See more keywords
CDC25B is required for the metaphase I-metaphase II transition in mouse oocytes.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of cell science"
DOI: 10.1242/jcs.252924
Abstract: Mammalian oocytes are arrested at meiotic prophase I. The dual-specificity phosphatase CDC25B is essential for cyclin-dependent kinase 1 (CDK1) activation that drives resumption of meiosis. CDC25B reverses the inhibitory effect of the protein kinases WEE1/MYT1… read more here.
Keywords: metaphase; cdc25b oocytes; cdk1 activation; cdc25b ... See more keywords
Interruption of an MSH4 homolog blocks meiosis in metaphase I and eliminates spore formation in Pleurotus ostreatus
Sign Up to like & getrecommendations! Published in 2020 at "PLoS ONE"
DOI: 10.1371/journal.pone.0241749
Abstract: Pleurotus ostreatus, one of the most widely cultivated edible mushrooms, produces high numbers of spores causing severe respiratory health problems for people, clogging of filters and spoilage of produce. A non-sporulating commercial variety (SPOPPO) has… read more here.
Keywords: metaphase; meiosis; spore formation; msh4 homolog ... See more keywords
Spindle shape and volume differ in high- and low-quality metaphase II oocytes.
Sign Up to like & getrecommendations! Published in 2024 at "Reproduction"
DOI: 10.1530/rep-23-0281
Abstract: The proper development of embryos strongly depends on the quality of oocytes, so the evaluation of oocytes may be a useful initial step in in vitro fertilization procedures. Additionally, it enables embryologists to make more… read more here.
Keywords: quality; shape volume; metaphase; mii ... See more keywords
Oligo-FISH Can Identify Chromosomes and Distinguish Hippophaë rhamnoides L. Taxa
Sign Up to like & getrecommendations! Published in 2022 at "Genes"
DOI: 10.3390/genes13020195
Abstract: Oligo-fluorescence in situ hybridization (FISH) facilitates precise chromosome identification and comparative cytogenetic analysis. Detection of autosomal chromosomes of Hippophaë rhamnoides has not been achieved using oligonucleotide sequences. Here, the chromosomes of five H. rhamnoides taxa… read more here.
Keywords: metaphase; oligo fish; rhamnoides taxa; mitotic metaphase ... See more keywords
siRNA-Mediated BmAurora B Depletion Impedes the Formation of Holocentric Square Spindles in Silkworm Metaphase BmN4 Cells
Sign Up to like & getrecommendations! Published in 2024 at "Insects"
DOI: 10.3390/insects15010072
Abstract: Simple Summary In this study, we explored the assembly of the mitotic spindle in silkworm ovary-derived BmN4 cells, with a specific focus on the role played by a key protein called BmAurora B. Using advanced… read more here.
Keywords: metaphase; mediated bmaurora; bmn4 cells; tubulin ... See more keywords