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Published in 2018 at "Scientific Reports"
DOI: 10.1038/s41598-018-26238-2
Abstract: Preparation of mouse embryonic fibroblast (MEF) feeder cells to maintain pluripotent stem cells (PSCs) is time consuming and involved in animal issues. Here, we demonstrated a novel method to prepare feeder cells with high efficiency,…
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Keywords:
methanol fixed;
cells maintain;
stem cells;
feeder cells ... See more keywords