Improved method for in situ biolistic transformation to analyze barley–powdery mildew interactions
Sign Up to like & getrecommendations! Published in 2017 at "Journal of General Plant Pathology"
DOI: 10.1007/s10327-017-0712-0
Abstract: Although the use of stable transformants is indispensable to elucidate mechanisms underlying molecular plant–pathogen interactions, this approach remains difficult to apply to crops. Alternatively, biolistic transformation has often been used as a transient expression method… read more here.
Keywords: situ biolistic; transformation; analyze; method situ ... See more keywords
Simplified Method for the In Situ Collection and Laboratory Analysis of Cosmogenic Tracers (Sulfur-35 and Sodium-22) to Determine Residence Time Distributions and Water Ages.
Sign Up to like & getrecommendations! Published in 2021 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.0c04490
Abstract: The use of cosmogenically produced sulfur-35 (T1/2 = 87 days) and sodium-22 (T1/2 = 2.6 years) as intrinsic tracers can provide valuable information on catchment hydrology, flow paths, and subsurface storage. A new and straightforward… read more here.
Keywords: water; chemistry; situ collection; method situ ... See more keywords
i-GONAD: a robust method for in situ germline genome engineering using CRISPR nucleases
Sign Up to like & getrecommendations! Published in 2018 at "Genome Biology"
DOI: 10.1186/s13059-018-1400-x
Abstract: We present a robust method called improved-Genome editing via Oviductal Nucleic Acids Delivery (i-GONAD) that delivers CRISPR ribonucleoproteins to E0.7 embryos via in situ electroporation. The method generates mouse models containing single-base changes, kilobase-sized deletions,… read more here.
Keywords: gonad robust; robust method; method; method situ ... See more keywords
A rapid preparation method for in situ nanomechanical TEM tensile specimens
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Materials Research"
DOI: 10.1557/s43578-021-00167-9
Abstract: Specimen preparation is a critical aspect in electron microscopy and is particularly challenging for in situ investigations that require specimens to fit onto specific testing devices. We report a modification of a substrate lift-off technique… read more here.
Keywords: method situ; situ nanomechanical; preparation method; tensile ... See more keywords
U-Pb (ID-TIMS) geochronological method and “in-situ” SIMS zircon dating: possibilities and constrains
Sign Up to like & getrecommendations! Published in 2020 at "Lithosphere"
DOI: 10.24930/1681-9004-2020-20-3-411-431
Abstract: Research subject and methods . The work is based on a comparative study of the data obtained by the authors and those published in literature on U-Pb ID-TIMS and “in-situ” isotopic examination of zircons extracted from… read more here.
Keywords: geochronological method; zircon; tims icp; method situ ... See more keywords
Bionanocellulose/Poly(Vinyl Alcohol) Composites Produced by In-Situ Method and Ex-Situ/Impregnation or Sterilization Methods
Sign Up to like & getrecommendations! Published in 2021 at "Materials"
DOI: 10.3390/ma14216340
Abstract: The purpose of the work was to obtain composites based on bionanocellulose (BNC) and poly(vinyl alcohol) (PVA) for specific biomedical and cosmetic applications and to determine how the method and conditions of their preparation affect… read more here.
Keywords: situ method; vinyl alcohol; method situ; situ ... See more keywords