Protein−Protein Interfaces Probed by Methyl Labeling and Proton‐Detected Solid‐State NMR Spectroscopy
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DOI: 10.1002/cphc.201800542
Abstract: Abstract Proton detection and fast magic‐angle spinning have advanced biological solid‐state NMR, allowing for the backbone assignment of complex protein assemblies with high sensitivity and resolution. However, so far no method has been proposed to… read more here.
Keywords: protein; solid state; state nmr; protein protein ... See more keywords
Improved strategy for isoleucine 1H/13C methyl labeling in Pichia pastoris
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DOI: 10.1007/s10858-019-00281-1
Abstract: Site specific methyl labeling combined with methyl TROSY offers a powerful NMR approach to study structure and dynamics of proteins and protein complexes of high molecular weight. Robust and cost-effective methods have been developed for… read more here.
Keywords: methyl; isoleucine; methyl labeling; pichia pastoris ... See more keywords