Integration of single-molecule detection with endonuclease IV-assisted signal amplification for sensitive DNA methylation assay.
Sign Up to like & getrecommendations! Published in 2021 at "Chemical communications"
DOI: 10.1039/d0cc08306b
Abstract: We demonstrate the development of a new fluorescent biosensor for sensitive DNA methylation assay by integrating single-molecule detection with endo IV-assisted signal amplification. This biosensor possesses the characteristics of good selectivity and high sensitivity with… read more here.
Keywords: sensitive dna; methylation; detection; methylation assay ... See more keywords
Self-Ligation-Free Next Generation Sequencing Adapters Applied to Methylation Assay
Sign Up to like & getrecommendations! Published in 2023 at "Journal of Biomedical Nanotechnology"
DOI: 10.1166/jbn.2023.3518
Abstract: In developing a bisulfite-free methylation assay with the use of restriction enzymes, self-ligation of next generation sequencing adapters (NGS-adapter) is a technological bottleneck to be overcome. In the experiments of this study, a variety of… read more here.
Keywords: self ligation; methylation; methylation assay; next generation ... See more keywords