Advances in the classification of pediatric brain tumors through DNA methylation profiling: From research tool to frontline diagnostic
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DOI: 10.1002/cncr.31583
Abstract: Despite significant improvements in pediatric brain tumor therapy and outcome, too many children still die of disease, and too many survivors experience significant sequelae as a result of conventional therapies. The molecular characterization of pediatric… read more here.
Keywords: methylation profiling; brain; biology; pediatric brain ... See more keywords
Multiplex DNA methylation profiling by ARMS-PCR for body fluid identification
Sign Up to like & getrecommendations! Published in 2019 at "Forensic Science International: Genetics Supplement Series"
DOI: 10.1016/j.fsigss.2019.10.189
Abstract: Abstract A set of CpG sites were detected and evaluated to identify body fluids based on DNA methylation using the amplification refractory mutation system-PCR (ARMS-PCR). In this research, two multiplex DNA methylation reactions composed of… read more here.
Keywords: methylation; arms pcr; methylation profiling; dna methylation ... See more keywords
Use of DNA Methylation Profiling in Translational Oncology.
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DOI: 10.1016/j.semcancer.2020.12.011
Abstract: DNA methylation is a highly regulated process that has a critical role in human development and homeostatic control of the cell. The number of genes affected by anomalous DNA methylation in cancer-associated pathways is swiftly… read more here.
Keywords: dna methylation; oncology; methylation profiling; cancer ... See more keywords
LINC-03. MOLECULAR CLASSIFICATION OF PAEDIATRIC MEDULLOBLASTOMA FROM FOUR TERTIARY CENTRES IN MALAYSIA: DIAGNOSTIC DILEMMA WITH CONVENTIONAL METHODS
Sign Up to like & getrecommendations! Published in 2020 at "Neuro-Oncology"
DOI: 10.1093/neuonc/noaa222.438
Abstract: Abstract OBJECTIVE To determine the prognostic significance of the four molecular subgroups of medulloblastoma (MB) among children in Malaysia. METHODS We assembled MB samples of children < 18 years between January 1999 and July 2017… read more here.
Keywords: molecular classification; paediatric medulloblastoma; classification paediatric; methylation profiling ... See more keywords
Adult intracranial ependymoma - relevance of DNA methylation profiling for diagnosis, prognosis and treatment.
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DOI: 10.1093/neuonc/noad030
Abstract: BACKGROUND A methylation-based classification of ependymoma has recently found broad application. However, the diagnostic advantage and implications for treatment decisions remain unclear. Here, we retrospectively evaluate the impact of surgery and radiotherapy on outcome after… read more here.
Keywords: ependymoma; methylation; methylation profiling; dna methylation ... See more keywords
DNA Methylation Profiling Identifies Distinct Clusters in Angiosarcomas
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DOI: 10.1158/1078-0432.ccr-19-2180
Abstract: Purpose: DNA methylation profiling has previously uncovered biologically and clinically meaningful subgroups within many tumor types, but was not yet performed in angiosarcoma. Angiosarcoma is a rare sarcoma with very heterogeneous clinical presentations, which may… read more here.
Keywords: angiosarcoma; methylation; methylation profiling; dna methylation ... See more keywords
Genetic Alterations Precede DNA Methylation Changes in Juvenile Myelomonocytic Leukemia
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DOI: 10.1182/blood-2020-143443
Abstract: Introduction Juvenile myelomonocytic leukemia (JMML) is a rare and highly aggressive myeloid malignancy of early childhood. Approximately 95% of patients have at least one mutation that leads to hyperactive RAS signaling. Both the presence of… read more here.
Keywords: methylation; diagnosis; jmml; methylation profiling ... See more keywords
Methylation profiling of hypomethylating agent response and treatment failure in pediatric and young adult MDS/AML.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Clinical Oncology"
DOI: 10.1200/jco.2020.38.15_suppl.e22502
Abstract: e22502Background: Hypomethylating agents (HMAs) have favorable toxicity profiles and anti-leukemia activity in adult AML/MDS. We previously reported similar activity in eight pediatric patients (Ph... read more here.
Keywords: profiling hypomethylating; adult; hypomethylating agent; methylation profiling ... See more keywords
Tumor DNA Methylation Profiling Is a Robust and Independent Prognostic Marker for Pediatric Patients With Adrenocortical Tumors
Sign Up to like & getrecommendations! Published in 2021 at "Journal of the Endocrine Society"
DOI: 10.1210/jendso/bvab048.200
Abstract: Pediatric adrenocortical tumors (pACTs) display complex genomic background and lack robust prognostic biomarkers. However, pACT methylation profiling was recently associated with patient prognosis. In order to evaluate whether tumor DNA methylation is associated with patient… read more here.
Keywords: profiling robust; adrenocortical tumors; tumor dna; methylation ... See more keywords
Introduction to Single-Cell DNA Methylation Profiling Methods
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DOI: 10.3390/biom11071013
Abstract: DNA methylation is an epigenetic mechanism that is related to mammalian cellular differentiation, gene expression regulation, and disease. In several studies, DNA methylation has been identified as an effective marker to identify differences between cells.… read more here.
Keywords: single cell; methylation; cell dna; methylation profiling ... See more keywords
Targeted Methylation Profiling of Single Laser-Capture Microdissected Post-Mortem Brain Cells by Adapted Limiting Dilution Bisulfite Pyrosequencing (LDBSP)
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms232415571
Abstract: A reoccurring issue in neuroepigenomic studies, especially in the context of neurodegenerative disease, is the use of (heterogeneous) bulk tissue, which generates noise during epigenetic profiling. A workable solution to this issue is to quantify… read more here.
Keywords: methylation; dilution bisulfite; methylation profiling; laser capture ... See more keywords