Methylation-Sensitive High Resolution Melting (MS-HRM).
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7481-8_28
Abstract: Methylation-Sensitive High Resolution Melting (MS-HRM) is an in-tube, PCR-based method to detect methylation levels at specific loci of interest. A unique primer design facilitates a high sensitivity of the assays enabling detection of down to… read more here.
Keywords: resolution melting; sensitive high; high resolution; methylation sensitive ... See more keywords
The Use of Methylation-Sensitive Multiplex Ligation-Dependent Probe Amplification for Quantification of Imprinted Methylation.
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DOI: 10.1007/978-1-4939-7768-0_6
Abstract: Imprinting disorders are a group of congenital diseases that can result from multiple mechanisms affecting imprinted gene dosage including cytogenetic aberration and epigenetic anomalies. Quantification of CpG methylation and correct copy-number calling is required for… read more here.
Keywords: methylation; probe; ligation; sensitive multiplex ... See more keywords
DNA methylation landscape of 16 canine somatic tissues by methylation-sensitive restriction enzyme-based next generation sequencing
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DOI: 10.1038/s41598-021-89279-0
Abstract: DNA methylation plays important functions in gene expression regulation that is involved in individual development and various diseases. DNA methylation has been well studied in human and model organisms, but only limited data exist in… read more here.
Keywords: methylation sensitive; methylation; dna methylation; cpg sites ... See more keywords
DNA methylation estimation using methylation-sensitive restriction enzyme bisulfite sequencing (MREBS)
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DOI: 10.1371/journal.pone.0214368
Abstract: Whole-genome bisulfite sequencing (WGBS) and reduced representation bisulfite sequencing (RRBS) are widely used for measuring DNA methylation levels on a genome-wide scale. Both methods have limitations: WGBS is expensive and prohibitive for most large-scale projects;… read more here.
Keywords: methylation; bisulfite sequencing; sensitive restriction; dna methylation ... See more keywords
Quantification of Unmethylated Insulin DNA Using Methylation Sensitive Restriction Enzyme Digital Polymerase Chain Reaction
Sign Up to like & getrecommendations! Published in 2022 at "Transplant International"
DOI: 10.3389/ti.2022.10167
Abstract: Assessment of specific β-cell death can be used to determine the quality and viability of pancreatic islets prior to transplantation and hence predict the suitability of the pancreas for isolation. Recently, several groups have demonstrated… read more here.
Keywords: ins dna; dna using; methylation sensitive; using methylation ... See more keywords