Detection of TET-Oxidized 5-Methylcytosine Bases by Capillary Gel Electrophoresis.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0876-0_11
Abstract: The oxidation activity of the mammalian ten-eleven translocation dioxygenase (TET) on 5-methylcytosine (5mC) of DNA is usually monitored by analytical methods such as dot blotting and liquid chromatography-mass spectrometry (LC-MS). Herein, we describe a high… read more here.
Keywords: gel electrophoresis; detection tet; methylcytosine; capillary gel ... See more keywords
Luminescence Sensing for Qualitative and Quantitative Detection of 5-Methylcytosine.
Sign Up to like & getrecommendations! Published in 2018 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.8b02842
Abstract: 5-Methylcytosine (5mC) is revealed as a heritable epigenetic modification in genomic DNA. It has been reported that cytosine/guanine dinucleotides (CpG) hypermethylation in the promoter regions of tumor suppressor genes is related with inappropriate gene silencing,… read more here.
Keywords: sensing qualitative; luminescence sensing; methylcytosine; qualitative quantitative ... See more keywords
5-Methylcytosine immunohistochemistry for predicting cutaneous melanoma prognosis
Sign Up to like & getrecommendations! Published in 2024 at "Scientific Reports"
DOI: 10.1038/s41598-024-58011-z
Abstract: There is a correlation between DNA methylation and the diseased stage and poor survival. 5-methylcytosine (5-mC) is one of the epigenetic modifications of bases that researchers focus on. Staining with 5-mC immunohistochemistry was used to… read more here.
Keywords: immunohistochemistry predicting; methylcytosine; cutaneous melanoma; methylcytosine immunohistochemistry ... See more keywords
Distinct characteristics of putative archaeal 5-methylcytosine RNA methyltransferases unveil their substrate specificities and evolutionary ancestries
Sign Up to like & getrecommendations! Published in 2024 at "Journal of Biomolecular Structure and Dynamics"
DOI: 10.1080/07391102.2024.2325670
Abstract: Abstract 5-Methylcytosine methyltransferases (m5C MTases) are known to be involved in the modification of RNA. Although these enzymes have been relatively well characterized in bacteria and eukarya, a complete understanding of the archaeal counterparts is… read more here.
Keywords: m5c mtases; putative rna; methylcytosine; rna ... See more keywords
An improved residual network using deep fusion for identifying RNA 5-methylcytosine sites
Sign Up to like & getrecommendations! Published in 2022 at "Bioinformatics"
DOI: 10.1093/bioinformatics/btac532
Abstract: MOTIVATION 5-Methylcytosine (m5C) is a crucial post-transcriptional modification. With the development of technology, it is widely found in various RNAs. Numerous studies have indicated that m5C plays an essential role in various activities of organisms,… read more here.
Keywords: residual network; deep fusion; methylcytosine; model ... See more keywords
Enhanced nucleosome assembly at CpG sites containing an extended 5-methylcytosine analogue
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac444
Abstract: Abstract Methylation of cytosine to 5-methylcytosine (mC) at CpG sites is a prevalent reversible epigenetic mark in vertebrates established by DNA methyltransferases (MTases); the attached methyl groups can alter local structure of DNA and chromatin… read more here.
Keywords: cpg sites; dna; nucleosome assembly; methylcytosine ... See more keywords
Demonstration of 5-Methylcytosine-Rich DNA Sequences in Chiroptera
Sign Up to like & getrecommendations! Published in 2017 at "Cytogenetic and Genome Research"
DOI: 10.1159/000475740
Abstract: 5-Methylcytosine-rich heterochromatic regions were demonstrated in metaphase chromosomes of 5 species of Chiroptera by indirect immunofluorescence using a monoclonal anti-5-methylcytosine antibody. These species belong to 4 genera and 2 families and are characterized by divergent… read more here.
Keywords: demonstration methylcytosine; methylcytosine; methylcytosine rich; rich dna ... See more keywords
Hyperglycemia affects global 5-methylcytosine and 5-hydroxymethylcytosine in blood genomic DNA through upregulation of SIRT6 and TETs
Sign Up to like & getrecommendations! Published in 2019 at "Clinical Epigenetics"
DOI: 10.1186/s13148-019-0660-y
Abstract: BackgroundAccumulating evidence suggests that epigenetic changes play key roles in the pathogenesis of type 2 diabetes mellitus (T2DM). However, the dynamic regulation of 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) in diabetic peripheral blood DNA remains to… read more here.
Keywords: hydroxymethylcytosine; genomic dna; sirt6 tets; methylcytosine ... See more keywords
Comprehensive Analysis of DNA 5-Methylcytosine and N6-Adenine Methylation by Nanopore Sequencing in Hepatocellular Carcinoma
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Cell and Developmental Biology"
DOI: 10.3389/fcell.2022.827391
Abstract: DNA methylation is a widespread epigenetic signal in human genome. With Nanopore technology, differential methylation modifications including 5-methylcytosine (5mC) and 6-methyladenine (6mA) can be identified. 5mC is the most important modification in mammals, although 6mA… read more here.
Keywords: methylation; hepatocellular carcinoma; dna; unstable methylation ... See more keywords
Role of 5-methylcytosine in determining the prognosis, tumor microenvironment, and applicability of precision medicine in patients with hepatocellular carcinoma
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Genetics"
DOI: 10.3389/fgene.2022.984033
Abstract: Background: 5-methylcytosine has a profound impact on the development and progression of hepatocellular carcinoma. The aim of this study was to investigate the usefulness of 5-methylcytosine in determining the prognosis, tumor microenvironment, and applicability of… read more here.
Keywords: hepatocellular carcinoma; prognosis; methylcytosine; methylcytosine score ... See more keywords