A self-correcting method for the measurement of free calcium and magnesium concentrations by 1H NMR.
Sign Up to like & getrecommendations! Published in 2019 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.9b03008
Abstract: A method for the direct measurement of free Ca2+ and Mg2+ concentrations in the range 1-100 mM by NMR spectroscopy is demonstrated. The method automatically corrects for the effect of ionic strength on the activity… read more here.
Keywords: ca2 mg2; measurement free; self correcting; free ca2 ... See more keywords
Physiological Mg2+ Conditions Significantly Alter the Inhibition of HIV-1 and HIV-2 Reverse Transcriptases by Nucleoside and Non-Nucleoside Inhibitors in Vitro.
Sign Up to like & getrecommendations! Published in 2017 at "Biochemistry"
DOI: 10.1021/acs.biochem.6b00943
Abstract: Reverse transcriptases (RTs) are typically assayed in vitro with 5-10 mM Mg2+, whereas the free Mg2+ concentration in cells is much lower. Artificially high Mg2+ concentrations used in vitro can misrepresent different properties of human… read more here.
Keywords: hiv; inhibition; non nucleoside; mg2 ... See more keywords