Evaluation of suitable reference genes for normalization of quantitative reverse transcription PCR analyses in Clavibacter michiganensis
Sign Up to like & getrecommendations! Published in 2019 at "MicrobiologyOpen"
DOI: 10.1002/mbo3.928
Abstract: Clavibacter michiganensis, the causal agent of bacterial canker of tomato, is a Gram‐positive bacterium and a model for studying plant diseases. The real‐time quantitative reverse transcription PCR (real‐time qRT‐PCR) assay is widely used to quantify… read more here.
Keywords: reference genes; suitable reference; michiganensis; gene expression ... See more keywords
First clinical case of KPC-3–producing Klebsiella michiganensis in Europe
Sign Up to like & getrecommendations! Published in 2019 at "New Microbes and New Infections"
DOI: 10.1016/j.nmni.2019.100516
Abstract: Klebsiella michiganensis is a newly emerging human pathogen. We describe a case of bloodstream infection in an immunocompromised patient. The pathogen was repeatedly isolated from blood and one rectal swab, and was identified using routine… read more here.
Keywords: clinical case; klebsiella michiganensis; klebsiella; first clinical ... See more keywords
Comparative transcriptome analysis of resistant and cultivated tomato lines in response to Clavibacter michiganensis subsp. michiganensis.
Sign Up to like & getrecommendations! Published in 2021 at "Genomics"
DOI: 10.1016/j.ygeno.2021.05.033
Abstract: Clavibacter michiganensis subsp. michiganensis (Cmm) is a gram-positive bacterium causing destructive bacterial wilt and canker disease in tomato. Herein, a comparative transcriptome analysis was performed on Cmm-resistant and -susceptible tomato lines. Tomato seedling were inoculated… read more here.
Keywords: transcriptome analysis; michiganensis; response; analysis ... See more keywords
The aphA1 kanamycin and neomycin resistance gene originated in Klebsiella michiganensis
Sign Up to like & getrecommendations! Published in 2025 at "Journal of Antimicrobial Chemotherapy"
DOI: 10.1093/jac/dkaf372
Abstract: Abstract Background The origins of several antibiotic resistance genes have been traced to intrinsic genes present in bacterial chromosomes. The aphA1 gene, which confers resistance to the aminoglycosides kanamycin and neomycin, is commonly found in… read more here.
Keywords: resistance; michiganensis; klebsiella michiganensis; gene ... See more keywords
Comparative genome analysis of tomato‐pathogenic Clavibacter michiganensis strains reveal genetic diversity in virulence genes
Sign Up to like & getrecommendations! Published in 2025 at "Annals of Applied Biology"
DOI: 10.1111/aab.70045
Abstract: Bacterial canker caused by Gram‐positive Clavibacter michiganensis subsp. michiganensis (Cmm) bacteria is a devastating disease of varied plant hosts. The host selection and pathogenicity are varied among different isolates based on genomic variations. Here, the… read more here.
Keywords: clavibacter michiganensis; pathogenicity; analysis; michiganensis ... See more keywords
Differentiation of Clavibacter michiganensis subsp. sepedonicus using PCR melting profile and variable number of tandem repeat methods
Sign Up to like & getrecommendations! Published in 2019 at "Letters in Applied Microbiology"
DOI: 10.1111/lam.13081
Abstract: The potato phytopathogen Clavibacter michiganensis subsp. sepedonicus (Cms) is a causative agent of bacterial ring rot, which is a serious threat to crops. In EU member countries, Cms is subject to quarantine and has to… read more here.
Keywords: tandem repeat; number tandem; variable number; michiganensis ... See more keywords
Bacterial alarmone (p)ppGpp mediates the pathogenicity of Clavibacter michiganensis via a dual mechanism that affects both enzyme production and the Tat secretion system
Sign Up to like & getrecommendations! Published in 2025 at "mSystems"
DOI: 10.1128/msystems.00135-25
Abstract: ABSTRACT The gram-positive bacterium Clavibacter michiganensis (Cm) causes bacterial canker of tomato. The infection process of Cm is of considerable interest, and the role of the alarmone guanosine tetraphosphate or pentaphosphate [(p)ppGpp], a global regulator,… read more here.
Keywords: clavibacter michiganensis; pathogenicity; michiganensis; secretion ... See more keywords
The Emerging Nosocomial Pathogen Klebsiella michiganensis: Genetic Analysis of a KPC-3 Producing Strain Isolated from Venus Clam
Sign Up to like & getrecommendations! Published in 2022 at "Microbiology Spectrum"
DOI: 10.1128/spectrum.04235-22
Abstract: Klebsiella michiganensis is an emerging nosocomial pathogen, and, so far, few studies describe isolates of clinical origin in the environment. This study contributes to the understanding of how the dissemination of carbapenem-resistance outside the hospital… read more here.
Keywords: emerging nosocomial; michiganensis; klebsiella michiganensis; klebsiella ... See more keywords
Multiplex real-time PCR for the detection of Clavibacter michiganensis subsp. michiganensis, Pseudomonas syringae pv. tomato and pathogenic Xanthomonas species on tomato plants
Sign Up to like & getrecommendations! Published in 2020 at "PLoS ONE"
DOI: 10.1371/journal.pone.0227559
Abstract: A multiplex real-time PCR method based on fluorescent TaqMan® probes was developed for the simultaneous detection of the tomato pathogenic bacteria Clavibacter michiganensis subsp. michiganensis, Pseudomonas syringae pv. tomato and bacterial spot-causing xanthomonads. The specificity… read more here.
Keywords: multiplex real; tomato pathogenic; michiganensis; real time ... See more keywords
Enrichment of Clavibacter michiganensis subsp. michiganensis in tomato seed extracts
Sign Up to like & getrecommendations! Published in 2017 at "Seed Science and Technology"
DOI: 10.15258/sst.2017.45.1.14
Abstract: An enrichment procedure was developed to improve detection of Clavibacter michiganensis subsp. michiganensis (Cmm) in tomato seed extracts. In the final procedure, seeds were soaked overnight in a buffer at 4°C and homogenised. Seed extracts… read more here.
Keywords: seed; michiganensis; cmm; seed extracts ... See more keywords
Epidemiology of Klebsiella michiganensis Carrying Multidrug-Resistant IncHI5 Plasmids in the Southeast Coastal Area of China
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DOI: 10.2147/idr.s358839
Abstract: Purpose This study aimed to explore the genomic characterization of multidrug-resistant IncHI5-carrying Klebsiella michiganensis strains and detailed genomic dissection of the IncHI5 plasmids. Materials and Methods Through whole-genome sequencing, the IncHI5 plasmid pK92-qnrS was obtained… read more here.
Keywords: multidrug resistant; inchi5 plasmids; michiganensis; resistant inchi5 ... See more keywords