Natural product discovery through microbial genome mining.
Sign Up to like & getrecommendations! Published in 2020 at "Current opinion in chemical biology"
DOI: 10.1016/j.cbpa.2020.07.010
Abstract: The advent of the genomic era has opened up enormous possibilities for the discovery of new natural products. Also known as specialized metabolites, these compounds produced by bacteria, fungi, and plants have long been sought… read more here.
Keywords: product discovery; biology; natural product; genome mining ... See more keywords
Efficient Single-Nucleotide Microbial Genome Editing Achieved Using CRISPR/Cpf1 with Maximally 3′-End-Truncated crRNAs
Sign Up to like & getrecommendations! Published in 2022 at "ACS Synthetic Biology"
DOI: 10.1021/acssynbio.2c00054
Abstract: Mismatch tolerance, a cause of the off-target effect, impedes accurate genome editing with the CRISPR/Cas system. Herein, we observed that oligonucleotide-directed single-base substitutions could be rarely introduced in the microbial genome using CRISPR/Cpf1-mediated negative selection.… read more here.
Keywords: genome editing; microbial genome; genome; end truncated ... See more keywords
Millstone: software for multiplex microbial genome analysis and engineering
Sign Up to like & getrecommendations! Published in 2017 at "Genome Biology"
DOI: 10.1186/s13059-017-1223-1
Abstract: Inexpensive DNA sequencing and advances in genome editing have made computational analysis a major rate-limiting step in adaptive laboratory evolution and microbial genome engineering. We describe Millstone, a web-based platform that automates genotype comparison and… read more here.
Keywords: engineering; analysis; engineering millstone; microbial genome ... See more keywords
Approaches for in silico finishing of microbial genome sequences
Sign Up to like & getrecommendations! Published in 2017 at "Genetics and Molecular Biology"
DOI: 10.1590/1678-4685-gmb-2016-0230
Abstract: Abstract The introduction of next-generation sequencing (NGS) had a significant effect on the availability of genomic information, leading to an increase in the number of sequenced genomes from a large spectrum of organisms. Unfortunately, due… read more here.
Keywords: silico finishing; genome sequences; approaches silico; microbial genome ... See more keywords
A Comparison of Microbial Genome Web Portals
Sign Up to like & getrecommendations! Published in 2019 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2019.00208
Abstract: Microbial genome web portals have a broad range of capabilities that address a number of information-finding and analysis needs for scientists. This article compares the capabilities of the major microbial genome web portals to aid… read more here.
Keywords: microbial genome; web portals; include genomic; genome web ... See more keywords