Site-Specific Antibody-Drug Conjugation Using Microbial Transglutaminase.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9546-2_8
Abstract: Antibody-drug conjugates (ADCs) are a relatively young class of cancer therapeutics that combine the superior selectivity of monoclonal antibodies (mAbs) with the high potency of cytotoxic agents. In the first generation of ADCs, the toxic… read more here.
Keywords: site specific; antibody drug; drug; conjugation ... See more keywords
Tailoring Activity and Selectivity of Microbial Transglutaminase.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9546-2_9
Abstract: Microbial transglutaminase (mTG), a protein-glutamine γ-glutamyltransferase from Streptomyces mobaraensis, is an enzyme capable of forming isopeptide bonds between the nearly inert (from the chemical point of view) γ-carboxamides present in the side chain of glutamine… read more here.
Keywords: transglutaminase; mtg; tailoring activity; activity selectivity ... See more keywords
Effect of introducing a disulfide bridge on the thermostability of microbial transglutaminase from Streptomyces mobaraensis.
Sign Up to like & getrecommendations! Published in 2021 at "Applied microbiology and biotechnology"
DOI: 10.1007/s00253-021-11200-6
Abstract: Microbial transglutaminase (MTG) has been used extensively in academic research and the food industry through cross-linking or posttranslational modification of proteins. In our previous paper, the activity-increased MTG mutants were obtained by means of rational… read more here.
Keywords: microbial transglutaminase; bridge; disulfide bridge; mtg ... See more keywords
Specific mutation of transglutaminase gene from Streptomyces hygroscopicus H197 and characterization of microbial transglutaminase
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Biosciences"
DOI: 10.1007/s12038-017-9707-4
Abstract: Abstract Microbial transglutaminase (MTG) gene (mtg) from Streptomyces hygroscopicus H197 strain was cloned by PCR and mutated by deleting a specific 84 bp fragment using overlapping extension PCR. The mutant MTG and the wild MTG genes… read more here.
Keywords: streptomyces hygroscopicus; transglutaminase; hygroscopicus h197; mtg ... See more keywords
Preparation and Characterization of a pH-responsive Polymer that Interacts with Microbial Transglutaminase during Affinity Precipitation
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology and Bioprocess Engineering"
DOI: 10.1007/s12257-017-0366-y
Abstract: Microbial transglutaminase (MTG) has been widely used in the food and pharmaceuticals industries. In this study, MTG was purified using affinity precipitation with an affinity polymer (PMMDN-T), which was synthesized using a pH-responsive polymer (PMMDN)… read more here.
Keywords: responsive polymer; affinity; affinity precipitation; polymer ... See more keywords
Effect of microbial transglutaminase on the mechanical properties and microstructure of acid-induced gels and emulsion gels produced from thermal denatured egg white proteins.
Sign Up to like & getrecommendations! Published in 2020 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2020.03.008
Abstract: In the current study, the impact of microbial transglutaminase (MTGase)-mediated crosslinking on the thermally denatured egg white protein (TD-EWP) made in alkaline pH in relation to their gelling properties of in gel and emulsion gel… read more here.
Keywords: mechanical properties; denatured egg; egg white; emulsion ... See more keywords
High-Affinity Aptamer Selection for Microbial Transglutaminase and Application in Colorimetric-SERS Dual-Mode Detection.
Sign Up to like & getrecommendations! Published in 2025 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.5c03609
Abstract: Microbial transglutaminase (MTGase) from Streptomyces mobaraensis is widely used in food processing to improve texture but poses health risks due to immune reactions and masking raw material quality. This study developed a sensitive detection method… read more here.
Keywords: detection; high affinity; affinity aptamer; dual mode ... See more keywords
Protein-Grafted Polymers Prepared Through a Site-Specific Conjugation by Microbial Transglutaminase for an Immunosorbent Assay.
Sign Up to like & getrecommendations! Published in 2017 at "Biomacromolecules"
DOI: 10.1021/acs.biomac.6b01538
Abstract: Protein-polymer conjugates have been developed in many fields. Most hybrids are composed of one protein attached to one or several polymer chains. The other form of hybrid involves the construction of multiple proteins on one… read more here.
Keywords: protein; site specific; polymer; specific conjugation ... See more keywords
Lactoferrin-Chitosan Composite Hydrogels Induced by Microbial Transglutaminase: Potential Delivery Systems for Thermosensitive Bioactive Substances.
Sign Up to like & getrecommendations! Published in 2024 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.4c01551
Abstract: In this work, lactoferrin (LF)-chitosan (CS) composite hydrogels with good loading capacity of thermosensitive bioactive substances were successfully obtained by microbial transglutaminase (MTG)-induced cross-linking. We evaluated the rheological, textural, and microstructural characteristics of the composite… read more here.
Keywords: bioactive substances; composite hydrogels; chitosan composite; lactoferrin chitosan ... See more keywords
Tag-free, specific conjugation of glycosylated IgG1 antibodies using microbial transglutaminase
Sign Up to like & getrecommendations! Published in 2022 at "RSC Advances"
DOI: 10.1039/d2ra05630e
Abstract: We present an efficient approach for tag-free, site-specific conjugation of a fully glycosylated antibody using microbial transglutaminase (mTG). We created variants of trastuzumab where a single surface-exposed residue of the human crystallizable fragment had been… read more here.
Keywords: using microbial; tag free; specific conjugation; microbial transglutaminase ... See more keywords
Microbial transglutaminase: An overview of recent applications in food and packaging
Sign Up to like & getrecommendations! Published in 2020 at "Biocatalysis and Biotransformation"
DOI: 10.1080/10242422.2020.1720660
Abstract: Abstract The glue of proteins, microbial transglutaminase (MTG) has been adopted in the food processing industries for its broad enzymatic action. Microorganisms such as Streptoverticillium and Streptomyces are the major sources, to decrease the cost… read more here.
Keywords: transglutaminase overview; overview recent; recent applications; transglutaminase ... See more keywords