Site-Specific Antibody-Drug Conjugation Using Microbial Transglutaminase.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9546-2_8
Abstract: Antibody-drug conjugates (ADCs) are a relatively young class of cancer therapeutics that combine the superior selectivity of monoclonal antibodies (mAbs) with the high potency of cytotoxic agents. In the first generation of ADCs, the toxic… read more here.
Keywords: site specific; antibody drug; drug; conjugation ... See more keywords
Tailoring Activity and Selectivity of Microbial Transglutaminase.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9546-2_9
Abstract: Microbial transglutaminase (mTG), a protein-glutamine γ-glutamyltransferase from Streptomyces mobaraensis, is an enzyme capable of forming isopeptide bonds between the nearly inert (from the chemical point of view) γ-carboxamides present in the side chain of glutamine… read more here.
Keywords: transglutaminase; mtg; tailoring activity; activity selectivity ... See more keywords
Effect of introducing a disulfide bridge on the thermostability of microbial transglutaminase from Streptomyces mobaraensis.
Sign Up to like & getrecommendations! Published in 2021 at "Applied microbiology and biotechnology"
DOI: 10.1007/s00253-021-11200-6
Abstract: Microbial transglutaminase (MTG) has been used extensively in academic research and the food industry through cross-linking or posttranslational modification of proteins. In our previous paper, the activity-increased MTG mutants were obtained by means of rational… read more here.
Keywords: microbial transglutaminase; bridge; disulfide bridge; mtg ... See more keywords
Specific mutation of transglutaminase gene from Streptomyces hygroscopicus H197 and characterization of microbial transglutaminase
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Biosciences"
DOI: 10.1007/s12038-017-9707-4
Abstract: Abstract Microbial transglutaminase (MTG) gene (mtg) from Streptomyces hygroscopicus H197 strain was cloned by PCR and mutated by deleting a specific 84 bp fragment using overlapping extension PCR. The mutant MTG and the wild MTG genes… read more here.
Keywords: streptomyces hygroscopicus; transglutaminase; hygroscopicus h197; mtg ... See more keywords
Preparation and Characterization of a pH-responsive Polymer that Interacts with Microbial Transglutaminase during Affinity Precipitation
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology and Bioprocess Engineering"
DOI: 10.1007/s12257-017-0366-y
Abstract: Microbial transglutaminase (MTG) has been widely used in the food and pharmaceuticals industries. In this study, MTG was purified using affinity precipitation with an affinity polymer (PMMDN-T), which was synthesized using a pH-responsive polymer (PMMDN)… read more here.
Keywords: responsive polymer; affinity; affinity precipitation; polymer ... See more keywords
Effect of microbial transglutaminase on the mechanical properties and microstructure of acid-induced gels and emulsion gels produced from thermal denatured egg white proteins.
Sign Up to like & getrecommendations! Published in 2020 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2020.03.008
Abstract: In the current study, the impact of microbial transglutaminase (MTGase)-mediated crosslinking on the thermally denatured egg white protein (TD-EWP) made in alkaline pH in relation to their gelling properties of in gel and emulsion gel… read more here.
Keywords: mechanical properties; denatured egg; egg white; emulsion ... See more keywords
Protein-Grafted Polymers Prepared Through a Site-Specific Conjugation by Microbial Transglutaminase for an Immunosorbent Assay.
Sign Up to like & getrecommendations! Published in 2017 at "Biomacromolecules"
DOI: 10.1021/acs.biomac.6b01538
Abstract: Protein-polymer conjugates have been developed in many fields. Most hybrids are composed of one protein attached to one or several polymer chains. The other form of hybrid involves the construction of multiple proteins on one… read more here.
Keywords: protein; site specific; polymer; specific conjugation ... See more keywords
Tag-free, specific conjugation of glycosylated IgG1 antibodies using microbial transglutaminase
Sign Up to like & getrecommendations! Published in 2022 at "RSC Advances"
DOI: 10.1039/d2ra05630e
Abstract: We present an efficient approach for tag-free, site-specific conjugation of a fully glycosylated antibody using microbial transglutaminase (mTG). We created variants of trastuzumab where a single surface-exposed residue of the human crystallizable fragment had been… read more here.
Keywords: using microbial; tag free; specific conjugation; microbial transglutaminase ... See more keywords
Microbial transglutaminase: An overview of recent applications in food and packaging
Sign Up to like & getrecommendations! Published in 2020 at "Biocatalysis and Biotransformation"
DOI: 10.1080/10242422.2020.1720660
Abstract: Abstract The glue of proteins, microbial transglutaminase (MTG) has been adopted in the food processing industries for its broad enzymatic action. Microorganisms such as Streptoverticillium and Streptomyces are the major sources, to decrease the cost… read more here.
Keywords: transglutaminase overview; overview recent; recent applications; transglutaminase ... See more keywords
Skim yoghurt with microbial transglutaminase: evaluation of consumer acceptance
Sign Up to like & getrecommendations! Published in 2019 at "CyTA - Journal of Food"
DOI: 10.1080/19476337.2019.1577304
Abstract: ABSTRACT The decrease in the fat content of yoghurt causes sensory modifications. Microbial transglutaminase (TG) has been proposed as an alternative for reducing the problems caused by the fat reduction. This work deals with the… read more here.
Keywords: acceptance; yoghurt; skim yoghurt; yoghurt microbial ... See more keywords
Beneficial Role of Microbial Transglutaminase in the Pathogenetic Mechanisms of Coeliac Disease
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Pediatric Gastroenterology and Nutrition"
DOI: 10.1097/mpg.0000000000003451
Abstract: ABSTRACT Coeliac disease (CD) is caused by immunological intolerance to wheat gluten and related proteins of rye and barley. Consequently, gluten-free (GF) products have been developed but technological implementation is required to improve their intrinsic… read more here.
Keywords: role; coeliac disease; transglutaminase; mtg ... See more keywords