Microdroplet PCR for Highly Multiplexed Targeted Bisulfite Sequencing.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7481-8_17
Abstract: Many methods exist for examining CpG DNA methylation. However, many of these are qualitative, laborious to apply to a large number of genes simultaneously, or are not easy to target to specific regions of interest.… read more here.
Keywords: pcr highly; bisulfite sequencing; highly multiplexed; microdroplet pcr ... See more keywords
Real-Time Quantifying Microdroplet Synthesis of Metal-Organic Framework Colloids Using Gas-Phase Electrophoresis.
Sign Up to like & getrecommendations! Published in 2023 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c05511
Abstract: A hyphenated electrospray-differential mobility analysis (ES-DMA) was developed for providing a high-resolution, real-time quantitative analysis on the metal-organic framework (MOF) colloids produced via the concept of microfluidic flow chemistry. Zeolitic imidazolate framework-8 was chosen as… read more here.
Keywords: microdroplet; chemistry; framework; real time ... See more keywords
Rapid Redox Cycling of Fe(II)/Fe(III) in Microdroplets during Iron-Citric Acid Photochemistry.
Sign Up to like & getrecommendations! Published in 2023 at "Environmental science & technology"
DOI: 10.1021/acs.est.2c07897
Abstract: Fe(III) and carboxylic acids are common compositions in atmospheric microdroplet systems like clouds, fogs, and aerosols. Although photochemical processes of Fe(III)-carboxylate complexes have been extensively studied in bulk aqueous solution, relevant information on the dynamic… read more here.
Keywords: acid photochemistry; microdroplet; iron citric; acid ... See more keywords
Generating Microdroplet Array on Photonic Pseudo-paper for Absolute Quantification of Nucleic Acids.
Sign Up to like & getrecommendations! Published in 2018 at "ACS applied materials & interfaces"
DOI: 10.1021/acsami.8b11552
Abstract: Here, we propose a new platform for digital nucleic acid assay based on a microdroplet array. The hydrophilic-superhydrophobic property of the pseudopaper photonic nitrocellulose substrate is employed to divide an aqueous sample containing the target… read more here.
Keywords: microdroplet array; array photonic; generating microdroplet; microdroplet ... See more keywords
Comb Polyelectrolytes Stabilize Complex Coacervate Microdroplet Dispersions.
Sign Up to like & getrecommendations! Published in 2022 at "ACS macro letters"
DOI: 10.1021/acsmacrolett.2c00327
Abstract: Complex coacervate microdroplets are membraneless compartments that selectively sequester biological molecules from their surroundings and enhance bioreactions. Yet, their use as protocell models and bioreactors has been limited owing to a lack of feasible strategies… read more here.
Keywords: microdroplet; complex coacervate; coacervate; polyelectrolytes stabilize ... See more keywords
Imaging of pH distribution inside individual microdroplet by stimulated Raman microscopy.
Sign Up to like & getrecommendations! Published in 2023 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2219588120
Abstract: Aerosol microdroplets as microreactors for many important atmospheric reactions are ubiquitous in the atmosphere. pH largely regulates the chemical processes within them; however, how pH and chemical species spatially distribute within an atmospheric microdroplet is… read more here.
Keywords: imaging distribution; microdroplet; microscopy; distribution inside ... See more keywords
Microdroplet Actuation via Light Line Optoelectrowetting (LL-OEW)
Sign Up to like & getrecommendations! Published in 2021 at "International Journal of Analytical Chemistry"
DOI: 10.1155/2021/3402411
Abstract: Meanwhile, electrowetting-on-dielectric (EWOD) is a well-known phenomenon, even often exploited in active micro-optics to change the curvature of microdroplet lenses or in analytical chemistry with digital microfluidics (DMF, lab on a chip 2.0) to move/actuate… read more here.
Keywords: chemistry; optoelectrowetting oew; light line; chip ... See more keywords
Laser-Induced Single Microdroplet Formation and Simultaneous Water-to-Single Microdroplet Extraction/Detection in Aqueous 1-Butanol Solutions
Sign Up to like & getrecommendations! Published in 2017 at "Bulletin of the Chemical Society of Japan"
DOI: 10.1246/bcsj.20160401
Abstract: Focused 1064-nm laser beam irradiation to an aqueous 1-butanol (BuOH) solution (7.1–7.4 wt % in H2O) resulted in formation of a single picoliter-volume BuOH droplet. Since water (H2O) absorbs 1064-nm laser light, an aqueous BuOH… read more here.
Keywords: single microdroplet; laser; water; aqueous butanol ... See more keywords
Tapered optical fiber waveguide coupling to whispering gallery modes of liquid crystal microdroplet for thermal sensing application.
Sign Up to like & getrecommendations! Published in 2017 at "Optics express"
DOI: 10.1364/oe.25.000918
Abstract: We demonstrate efficient coupling to the optical whispering gallery modes (WGMs) of nematic liquid crystal (NLC) microdroplets immersed in an immiscible aqueous environment. An individual NLC microdroplet, confined at the tip of a microcapillary, was… read more here.
Keywords: gallery modes; liquid crystal; whispering gallery; microdroplet ... See more keywords
Accessible, fast and easy fabrication of hydrophilic-in-hydrophobic microdroplet arrays
Sign Up to like & getrecommendations! Published in 2022 at "PLoS ONE"
DOI: 10.1371/journal.pone.0263282
Abstract: Microdroplet arrays (MDAs) are powerful tools for digital immunoassays, high-throughput screening and single cell analysis. However, MDAs are usually produced with cleanroom processes, which are associated with high costs and low availability. Furthermore, in order… read more here.
Keywords: microdroplet arrays; fabrication hydrophilic; microdroplet; accessible fast ... See more keywords
In Vitro Evaluation of Lipopolyplexes for Gene Transfection: Comparing 2D, 3D and Microdroplet-Enabled Cell Culture
Sign Up to like & getrecommendations! Published in 2020 at "Molecules"
DOI: 10.3390/molecules25143277
Abstract: Complexes combining nucleic acids with lipids and polymers (lipopolyplexes) show great promise for gene therapy since they enable compositional, physical and functional versatility to be optimized for therapeutic efficiency. When developing lipopolyplexes for gene delivery,… read more here.
Keywords: transfection; cell culture; microdroplet; culture ... See more keywords