An affordable plasmid miniprep suitable for proficient microinjection in Caenorhabditis elegans
Sign Up to like & getrecommendations! Published in 2020 at "3 Biotech"
DOI: 10.1007/s13205-020-02346-7
Abstract: A variety of techniques, including CRISPR-Cas9 genome editing, have been developed to produce genetically modified cell lines and animal models. In many cases, the success of the genome-editing techniques is dependent on the quality of… read more here.
Keywords: affordable plasmid; caenorhabditis elegans; microinjection; plasmid miniprep ... See more keywords
Electrical-assisted microinjection for analysis of fertilization and cell division in mammalian oocytes and early embryos.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in cell biology"
DOI: 10.1016/bs.mcb.2018.03.036
Abstract: Microinjection is an essential approach in the study of mammalian oocytes and early embryos, and is useful for the introduction of many molecules and reagents. Whereas microinjection into germinal vesicle stage oocytes is relatively simple… read more here.
Keywords: electrical assisted; oocytes early; microinjection; early embryos ... See more keywords
Cholinergic/opioid interaction in anterior cingulate cortex reduces the nociceptive response of vocalization in guinea pigs
Sign Up to like & getrecommendations! Published in 2017 at "Brain Research"
DOI: 10.1016/j.brainres.2017.07.013
Abstract: The anterior cingulate cortex (ACC) is crucial in the modulation of the sensory, affective and cognitive aspects of nociceptive processing. Also, it participates in the planning and execution of behavioral responses evoked by nociceptive stimuli… read more here.
Keywords: cingulate cortex; microinjection; anterior cingulate; vocalization ... See more keywords
Optimization of protocols for microinjection-based delivery of cryoprotective agents into Japanese whiting Sillago japonica embryos.
Sign Up to like & getrecommendations! Published in 2018 at "Cryobiology"
DOI: 10.1016/j.cryobiol.2018.10.007
Abstract: Microinjection has proven useful for introduction of low-permeability cryoprotective agents (CPAs) into fish eggs or embryos for cryopreservation. In this work, we examined the suitable conditions for single or combined microinjection into the perivitelline space… read more here.
Keywords: embryos; japanese whiting; protocols microinjection; microinjection ... See more keywords
Paradoxical anesthesia: Sleep-like EEG during anesthesia induced by mesopontine microinjection of GABAergic agents
Sign Up to like & getrecommendations! Published in 2021 at "Experimental Neurology"
DOI: 10.1016/j.expneurol.2021.113760
Abstract: General anesthetic agents are thought to induce loss-of-consciousness (LOC) and enable pain-free surgery by acting on the endogenous brain circuitry responsible for sleep-wake cycling. In clinical use, the entire CNS is exposed to anesthetic molecules… read more here.
Keywords: anesthesia induced; anesthesia; wake; microinjection ... See more keywords
The Next MacGyver: A Platform to Study Intestinal Organoids Using High-Throughput Computer-Driven Microinjection
Sign Up to like & getrecommendations! Published in 2018 at "Cellular and Molecular Gastroenterology and Hepatology"
DOI: 10.1016/j.jcmgh.2018.06.010
Abstract: he complex microbial community that lives in the Tcolon plays an important role in diverse biological pathways and is linked to many diseases including inflammatory bowel disease, obesity, and cancer. Defining how microbial interactions influence… read more here.
Keywords: throughput; gastroenterology; organoid cultures; microinjection ... See more keywords
Delivery of maize mosaic virus to planthopper vectors by microinjection increases infection efficiency and facilitates functional genomics experiments in the vector.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of virological methods"
DOI: 10.1016/j.jviromet.2019.05.010
Abstract: The corn planthopper, Peregrinus maidis, not only causes direct damage to plants by feeding, but also transmits maize mosaic virus (MMV) to the plant hosts. The virus is transmitted in a propagative manner but the… read more here.
Keywords: insects; vector; maize mosaic; microinjection ... See more keywords
Hypotensive effect induced by microinjection of Alamandine, a derivative of angiotensin-(1–7), into caudal ventrolateral medulla of 2K1C hypertensive rats
Sign Up to like & getrecommendations! Published in 2017 at "Peptides"
DOI: 10.1016/j.peptides.2017.09.005
Abstract: In the present study we evaluated the cardiovascular effects produced by microinjection of the new component of the renin-angiotensin system, alamandine, into caudal ventrolateral medulla of urethane-anesthetized normotensive and hypertensive 2K1C rats. The participation of… read more here.
Keywords: caudal ventrolateral; alamandine; angiotensin; microinjection ... See more keywords
Microinjection System to Enable Real-Time 3D Image Presentation Through Focal Position Adjustment
Sign Up to like & getrecommendations! Published in 2021 at "IEEE Robotics and Automation Letters"
DOI: 10.1109/lra.2021.3067857
Abstract: The microinjection technique, in which DNA is injected into cells, is widely applied in fields such as biology, medicine, and pharmacy. However, the implementation of the microinjection process requires skill and, thus, it is necessary… read more here.
Keywords: system; time image; real time; microinjection system ... See more keywords
Design and Implementation of an Automatic Batch Microinjection System for Zebrafish Larvae
Sign Up to like & getrecommendations! Published in 2022 at "IEEE Robotics and Automation Letters"
DOI: 10.1109/lra.2022.3143286
Abstract: Microinjection of zebrafish larvae is one of the most common procedures in biomedical experiments. Majority of microinjection work of zebrafish larvae in the laboratory is operated manually. Manual operation is influenced by many factors, and… read more here.
Keywords: microinjection system; zebrafish larvae; batch microinjection; microinjection ... See more keywords
Design and Development of an Automatic Microinjection System for High-Throughput Injection of Zebrafish Larvae
Sign Up to like & getrecommendations! Published in 2022 at "IEEE Transactions on Automation Science and Engineering"
DOI: 10.1109/tase.2021.3119405
Abstract: The microinjection of zebrafish larvae is a complicated work due to their small size and shape variations. In this article, for the first time, we design an automatic microinjection system dedicated to high-throughput injection of… read more here.
Keywords: injection; system; microinjection; zebrafish larvae ... See more keywords