Screening of Fungi Isolates for C-4 Hydroxylation of R-2-Phenoxypropionic Acid Based on a Novel 96-Well Microplate Assay Method
Sign Up to like & getrecommendations! Published in 2020 at "Applied Biochemistry and Biotechnology"
DOI: 10.1007/s12010-020-03303-z
Abstract: R-2-(4-Hydroxyphenoxy)propionic acid (R-HPPA) is a pivotal intermediate for the synthesis of aryloxyphenoxypropionate (APP) herbicide. To rapidly screen microbial isolates with the capacity of hydroxylating R-2-phenoxypropionic acid to R-HPPA from various environmental samples, a convenient and… read more here.
Keywords: assay method; hydroxylation; well microplate; microplate assay ... See more keywords
Application of a robust microplate assay to determine induced β-1,3-glucanase and chitinase activity in the cotton plant.
Sign Up to like & getrecommendations! Published in 2021 at "BioTechniques"
DOI: 10.2144/btn-2020-0015
Abstract: Resistance is induced in cotton plants as the result of either viral infection or exogenous application of elicitors. Induced resistance can be evaluated by determining the production of β-1,3-glucanase and chitinase in plants as a… read more here.
Keywords: glucanase chitinase; chitinase; application; microplate assay ... See more keywords
Developing a fluorometric urease activity microplate assay suitable for automated microbioreactor experiments
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2022.936759
Abstract: Quantifying urease activity is an important task for Microbial Induced Calcite Precipitation research. A new urease activity microplate assay using a fluorescent pH indicator is presented. The method is also suitable for automated measurements during… read more here.
Keywords: microplate assay; activity; urease activity; microbioreactor ... See more keywords
Probing Cell Redox State and Glutathione-Modulating Factors Using a Monochlorobimane-Based Microplate Assay
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DOI: 10.3390/antiox11020391
Abstract: Thiol compounds including predominantly glutathione (GSH) are key components of redox homeostasis, which are involved in the protection and regulation of mammalian cells. The assessment of cell redox status by means of in situ analysis… read more here.
Keywords: cell; cell redox; microplate assay; modulating factors ... See more keywords