Use of Microscale Thermophoresis (MST) to Measure Binding Affinities of Components of the Fusion Machinery.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8760-3_11
Abstract: Microscale thermophoresis is a relatively new technique used by an increasing number of academic laboratories to estimate relative binding affinities between ligand (analyte) that is titrated and a target (generally protein) that is either fluorescently… read more here.
Keywords: thermophoresis; binding affinities; microscale thermophoresis; fusion machinery ... See more keywords
Sensitive Microscale Thermophoresis Assay Using Aptamer Thermal Switch.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c03068
Abstract: Rapid sensitive detection of targets is desired in broad fields, such as environmental monitoring, food safety, and molecular diagnosis. We demonstrated an innovative strategy of constructing aptamer thermal switch for target detection, which affords a… read more here.
Keywords: heating induced; microscale thermophoresis; thermal switch; aptamer thermal ... See more keywords
Solution composition dependent Soret coefficient using commercial MicroScale Thermophoresis instrument
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DOI: 10.1039/d3ra00154g
Abstract: Thermal diffusion of particles in dilute aqueous suspensions is driven by the interactions between the dispersing medium and the particle, which are largely influenced by the properties of the medium. Using a commercial instrument to… read more here.
Keywords: microscale thermophoresis; soret coefficient; composition; thermophoresis ... See more keywords
Combining nano-differential scanning fluorimetry and microscale thermophoresis to investigate VDAC1 interaction with small molecules
Sign Up to like & getrecommendations! Published in 2023 at "Journal of Enzyme Inhibition and Medicinal Chemistry"
DOI: 10.1080/14756366.2022.2121821
Abstract: Abstract The mitochondrial voltage-dependent anion channel 1 (VDAC1) plays a central role in metabolism and apoptosis, which makes it a promising therapeutic target. Nevertheless, molecular mechanisms governing VDAC1 functioning remain unclear. Small-molecule ligands specifically interacting… read more here.
Keywords: vdac1; microscale thermophoresis; fluorimetry microscale; differential scanning ... See more keywords
Microscale Thermophoresis as a Tool to Study Protein Interactions and Their Implication in Human Diseases
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms23147672
Abstract: The review highlights how protein–protein interactions (PPIs) have determining roles in most life processes and how interactions between protein partners are involved in various human diseases. The study of PPIs and binding interactions as well… read more here.
Keywords: thermophoresis tool; microscale thermophoresis; human diseases; protein interactions ... See more keywords
Microscale Thermophoresis and Molecular Modelling to Explore the Chelating Drug Transportation in the Milk to Infant
Sign Up to like & getrecommendations! Published in 2022 at "Molecules"
DOI: 10.3390/molecules27144604
Abstract: The microscale thermophoresis (MST) technique was utilized to investigate lactoferrin–drug interaction with the iron chelator, deferiprone, using label-free system. MST depends on the intrinsic fluorescence of one interacting partner. The results indicated a significant interaction… read more here.
Keywords: interaction; microscale thermophoresis; pocket; milk ... See more keywords
The First Online Capillary Electrophoresis-Microscale Thermophoresis (CE-MST) Method for the Analysis of Dynamic Equilibria—The Determination of the Acidity Constant of Fluorescein Isothiocyanate
Sign Up to like & getrecommendations! Published in 2022 at "Molecules"
DOI: 10.3390/molecules27155010
Abstract: This article presents the first successful application of a capillary electrophoresis-microscale thermophoresis tandem technique (CE-MST) for determining the values of equilibrium constant, realized by connecting online the CE and MST instruments using a fused-silica capillary.… read more here.
Keywords: electrophoresis microscale; microscale thermophoresis; mst; acidity ... See more keywords