Microscopy‐based assay for semi‐quantitative detection of SARS‐CoV‐2 specific antibodies in human sera
Sign Up to like & getrecommendations! Published in 2020 at "Bioessays"
DOI: 10.1002/bies.202000257
Abstract: Emergence of the novel pathogenic coronavirus SARS‐CoV‐2 and its rapid pandemic spread presents challenges that demand immediate attention. Here, we describe the development of a semi‐quantitative high‐content microscopy‐based assay for detection of three major classes… read more here.
Keywords: microscopy; cov specific; microscopy based; sars cov ... See more keywords
Quantitative microscopy based on single-molecule fluorescence.
Sign Up to like & getrecommendations! Published in 2017 at "Current opinion in chemical biology"
DOI: 10.1016/j.cbpa.2017.06.004
Abstract: Quantitative microscopy is needed to understand reactions or phenomena carried out by biological molecules such as enzymes, receptors, and membrane-localized proteins. Counting the biomolecules of interest in single organelles or cellular compartments is critical in… read more here.
Keywords: single molecule; fluorescence; microscopy; microscopy based ... See more keywords
Structured illumination microscopy based on fiber devices
Sign Up to like & getrecommendations! Published in 2020 at "Optics Communications"
DOI: 10.1016/j.optcom.2019.125099
Abstract: We present a simple and compact approach of structured illumination microscopy by using three $2\times2$ fiber couplers and one $1\times4$ MEMS optics switch. One uniform and three fringe illumination patterns were produced by placing seven… read more here.
Keywords: optics; microscopy; illumination microscopy; microscopy based ... See more keywords
Light-microscopy-based connectomic reconstruction of mammalian brain tissue
Sign Up to like & getrecommendations! Published in 2025 at "Nature"
DOI: 10.1038/s41586-025-08985-1
Abstract: The information-processing capability of the brain’s cellular network depends on the physical wiring pattern between neurons and their molecular and functional characteristics. Mapping neurons and resolving their individual synaptic connections can be achieved by volumetric… read more here.
Keywords: brain; microscopy based; synapse level; brain tissue ... See more keywords
Atomic force microscopy-based single-molecule force spectroscopy detects DNA base mismatches.
Sign Up to like & getrecommendations! Published in 2019 at "Nanoscale"
DOI: 10.1039/c9nr05234h
Abstract: Atomic force microscopy-based single-molecule-force spectroscopy is limited by low throughput. We introduce addressable DNA origami to study multiple target molecules. Six target DNAs that differed by only a single base-pair mismatch were clearly differentiated a… read more here.
Keywords: microscopy; force microscopy; microscopy based; atomic force ... See more keywords
Fluorescence microscopy-based quantitation of GLUT4 translocation
Sign Up to like & getrecommendations! Published in 2022 at "Methods and Applications in Fluorescence"
DOI: 10.1088/2050-6120/ac4998
Abstract: Postprandial insulin-stimulated glucose uptake into target tissue is crucial for the maintenance of normal blood glucose homeostasis. This step is rate-limited by the number of facilitative glucose transporters type 4 (GLUT4) present in the plasma… read more here.
Keywords: translocation; microscopy based; microscopy; glut4 translocation ... See more keywords
X‐ray diffraction microscopy based on refractive optics
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Applied Crystallography"
DOI: 10.1107/s1600576717011037
Abstract: A formalism is presented for dark-field X-ray microscopy using refractive optics. The new technique can produce three-dimensional maps of lattice orientation and axial strain within millimetre-sized sampling volumes and is particularly suited to in situ… read more here.
Keywords: diffraction microscopy; refractive optics; ray diffraction; microscopy ... See more keywords
Innovative optofluidics and microscopy-based rapid analysis of pathogens.
Sign Up to like & getrecommendations! Published in 2020 at "Biomedical optics express"
DOI: 10.1364/boe.396345
Abstract: The timely knowledge and prescription of the most suitable antibiotic to treat bacterial infections is critical for the recovery of patients battling life-threatening bacterial infections. Unfortunately, current standard-of-care approaches relies on the empiric prescription of… read more here.
Keywords: microscopy; microscopy based; rapid analysis; based rapid ... See more keywords
Electrically-switched differential microscopy based on computing liquid-crystal platforms
Sign Up to like & getrecommendations! Published in 2024 at "Nanophotonics"
DOI: 10.1515/nanoph-2023-0688
Abstract: Abstract Detection of transparent phase specimens especially biological cells with desired contrasts is of great importance in visual display and medical diagnosis. Due to the pure-phase nature, conventional detection approaches may damage samples or require… read more here.
Keywords: liquid crystal; microscopy; microscopy based; computing liquid ... See more keywords
An inexpensive, customizable microscopy system for the automated quantification and characterization of multiple adherent cell types
Sign Up to like & getrecommendations! Published in 2018 at "PeerJ"
DOI: 10.7717/peerj.4937
Abstract: Cell quantification assays are essential components of most biological and clinical labs. However, many currently available quantification assays, including flow cytometry and commercial cell counting systems, suffer from unique drawbacks that limit their overall efficacy.… read more here.
Keywords: microscopy; microscopy based; based cytometer; cell types ... See more keywords