Flow Cytometry and Confocal Microscopy for ROS Evaluation in Fish and Human Spermatozoa.
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DOI: 10.1007/978-1-0716-0896-8_8
Abstract: Reactive oxygen species (ROS) could have a negative impact on sperm cellular function and viability. This chapter describes a protocol for oxidative stress evaluation using dichlorofluorescein (DCF) which can specifically reveal intracellular reactive oxygen species.… read more here.
Keywords: cytometry confocal; microscopy; microscopy ros; flow cytometry ... See more keywords