Binding of alpha‐fodrin to gamma‐tubulin accounts for its role in the inhibition of microtubule nucleation
Sign Up to like & getrecommendations! Published in 2019 at "FEBS Letters"
DOI: 10.1002/1873-3468.13425
Abstract: Non‐erythroid spectrin or fodrin is present as part of the γ‐tubulin ring complex (γ‐TuRC) in brain tissue and brain derived cells. Here, we show that fodrin, which is otherwise known for providing structural support to… read more here.
Keywords: microtubule nucleation; nucleation; gamma tubulin; tubulin ... See more keywords
Assaying Microtubule Nucleation.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0219-5_11
Abstract: Assaying microtubule nucleation is essential to understand the organization of microtubule networks in any cell type. In this chapter we describe methods for measuring nucleation activity at centrosomes and at mitotic chromatin in cell lines,… read more here.
Keywords: microtubule nucleation; microtubule; methodology; assaying microtubule ... See more keywords
Reconstitution of Microtubule Nucleation In Vitro Reveals Novel Roles for Mzt1
Sign Up to like & getrecommendations! Published in 2019 at "Current Biology"
DOI: 10.1016/j.cub.2019.05.058
Abstract: Summary Microtubule (MT) nucleation depends on the γ-tubulin complex (γ-TuC), in which multiple copies of the heterotetrameric γ-tubulin small complex (γ-TuSC) associate to form a ring-like structure (in metazoans, γ-tubulin ring complex; γ-TuRC) [1, 2,… read more here.
Keywords: microtubule nucleation; microtubule; mzt1; reconstitution ... See more keywords
Kinesin-5 Promotes Microtubule Nucleation and Assembly by Stabilizing a Lattice-Competent Conformation of Tubulin
Sign Up to like & getrecommendations! Published in 2019 at "Current Biology"
DOI: 10.1016/j.cub.2019.05.075
Abstract: Besides sliding apart antiparallel microtubules during spindle elongation, the mitotic kinesin-5, Eg5, promotes microtubule polymerization, emphasizing its importance in mitotic spindle length control. Here, we characterize the Eg5 microtubule polymerase mechanism by assessing motor-induced changes… read more here.
Keywords: microtubule nucleation; conformation tubulin; promotes microtubule; kinesin ... See more keywords
Phase separation of TPX2 enhances and spatially coordinates microtubule nucleation
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DOI: 10.1038/s41467-019-14087-0
Abstract: Phase separation of substrates and effectors is proposed to enhance biological reaction rates and efficiency. Targeting protein for Xklp2 (TPX2) is an effector of branching microtubule nucleation in spindles and functions with the substrate tubulin… read more here.
Keywords: microtubule nucleation; phase separation; tpx2;
Insights into the assembly and activation of the microtubule nucleator γ-TuRC
Sign Up to like & getrecommendations! Published in 2019 at "Nature"
DOI: 10.1038/s41586-019-1896-6
Abstract: Microtubules are dynamic polymers of α- and β-tubulin and have crucial roles in cell signalling, cell migration, intracellular transport and chromosome segregation 1 . They assemble de novo from αβ-tubulin dimers in an essential process… read more here.
Keywords: microtubule nucleation; microtubule; microscopy; turc ... See more keywords
Microtubule nucleation complex behavior is critical for cortical array homogeneity and xylem wall patterning.
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2203900119
Abstract: Significance The plant cortical microtubule array is an established model system for self-organization, with a rich history of complementary experiments, computer simulations, and analytical theory. A hitherto unappreciated question is how array homogeneity is maintained… read more here.
Keywords: wall; array homogeneity; nucleation; microtubule nucleation ... See more keywords
Identification and characterization of the land-plant-specific microtubule nucleation factor MACET4
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Cell Science"
DOI: 10.1242/jcs.232819
Abstract: ABSTRACT Here, we show that the embryophyte (land-plant)-specific protein MACERATOR4 (MACET4) binds microtubules in vitro and in vivo, promotes microtubule polymerization at sub-critical tubulin concentrations, decreases the lag phase in microtubule bulk polymerization assays, and… read more here.
Keywords: microtubule nucleation; microtubule; macet4; plant specific ... See more keywords
The ciliopathy protein CCDC66 controls mitotic progression and cytokinesis by promoting microtubule nucleation and organization
Sign Up to like & getrecommendations! Published in 2022 at "PLoS Biology"
DOI: 10.1371/journal.pbio.3001708
Abstract: Precise spatiotemporal control of microtubule nucleation and organization is critical for faithful segregation of cytoplasmic and genetic material during cell division and signaling via the primary cilium in quiescent cells. Microtubule-associated proteins (MAPs) govern assembly,… read more here.
Keywords: nucleation; ccdc66; nucleation organization; microtubule nucleation ... See more keywords
Biochemical reconstitution of branching microtubule nucleation
Sign Up to like & getrecommendations! Published in 2020 at "eLife"
DOI: 10.7554/elife.49797
Abstract: Microtubules are nucleated from specific locations at precise times in the cell cycle. However, the factors that constitute these microtubule nucleation pathways and their mode of action still need to be identified. Using purified Xenopus… read more here.
Keywords: nucleation; microtubule nucleation; biochemical reconstitution; reconstitution branching ... See more keywords