Articles with "minflux" as a keyword



Photoactivatable Xanthone (PaX) Dyes Enable Quantitative, Dual Color, and Live‐Cell MINFLUX Nanoscopy

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Published in 2024 at "Small Methods"

DOI: 10.1002/smtd.202301497

Abstract: The single‐molecule localization concept MINFLUX has triggered a reevaluation of the features of fluorophores for attaining nanometer‐scale resolution. MINFLUX nanoscopy benefits from temporally controlled fluorescence (“on”/“off”) photoswitching. Combined with an irreversible switching behavior, the localization… read more here.

Keywords: live cell; minflux; minflux nanoscopy; dual color ... See more keywords

Polarized ATP synthase in synaptic mitochondria induced by learning and plasticity signals

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Published in 2025 at "Communications Biology"

DOI: 10.1038/s42003-025-08963-3

Abstract: MINFLUX is a cutting-edge single-molecule localization microscopy technology that surpasses the conventional diffraction limit, enabling nanostructure visualization with exceptional precision. However, its application has largely been limited to cultured cells. In this research, we refined… read more here.

Keywords: minflux; dendritic spines; plasticity; atp synthase ... See more keywords

MINFLUX imaging of a bacterial molecular machine at nanometer resolution

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Published in 2022 at "Methods and Applications in Fluorescence"

DOI: 10.1088/2050-6120/aca880

Abstract: The resolution achievable with the established super-resolution fluorescence nanoscopy methods, such as STORM or STED, is in general not sufficient to resolve protein complexes or even individual proteins. Recently, minimal photon flux (MINFLUX) nanoscopy has… read more here.

Keywords: minflux; molecular machine; resolution; bacterial molecular ... See more keywords

Resolving the molecular architecture of the photoreceptor active zone with 3D-MINFLUX

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Published in 2022 at "Science Advances"

DOI: 10.1126/sciadv.abl7560

Abstract: Cells assemble macromolecular complexes into scaffoldings that serve as substrates for catalytic processes. Years of molecular neurobiology research indicate that neurotransmission depends on such optimization strategies. However, the molecular topography of the presynaptic active zone… read more here.

Keywords: active zone; architecture photoreceptor; minflux; zone ... See more keywords