Production of Minicircle DNA Vectors Using Site-Specific Recombinases.
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DOI: 10.1007/978-1-4939-7169-5_20
Abstract: Minicircle DNA vectors are plasmid derivatives free of bacterial elements. These vectors are mostly provided from common plasmids via recombination by site-specific recombinases in E. coli. Absence of bacterial backbone in minicircle vectors results in… read more here.
Keywords: minicircle dna; site specific; minicircle; specific recombinases ... See more keywords
Guide RNA Repertoires in the Main Lineages of Trypanosoma cruzi: High Diversity and Variable Redundancy Among Strains
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DOI: 10.3389/fcimb.2021.663416
Abstract: Trypanosoma cruzi, as other kinetoplastids, has a complex mechanism of editing of mitochondrial mRNAs that requires guide RNAs (gRNAs) coded in DNA minicircles in the kinetoplast. There are many variations on this mechanism among species.… read more here.
Keywords: among strains; minicircle; grna classes; redundancy ... See more keywords
Minicircle Mediated Gene Delivery to Canine and Equine Mesenchymal Stem Cells
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DOI: 10.3390/ijms18040819
Abstract: Gene-directed tissue repair offers the clinician, human or veterinary, the chance to enhance cartilage regeneration and repair at a molecular level. Non-viral plasmid vectors have key biosafety advantages over viral vector systems for regenerative therapies… read more here.
Keywords: vector; transfection; canine equine; minicircle ... See more keywords
Development of Minicircle Vectors Encoding COL7A1 Gene with Human Promoters for Non-Viral Gene Therapy for Recessive Dystrophic Epidermolysis Bullosa
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DOI: 10.3390/ijms222312774
Abstract: Recessive dystrophic epidermolysis bullosa (RDEB) is a rare autosomal inherited skin disorder caused by mutations in the COL7A1 gene that encodes type VII collagen (C7). The development of an efficient gene replacement strategy for RDEB… read more here.
Keywords: epidermolysis bullosa; non viral; gene; minicircle ... See more keywords