Minicircle DNA Vaccine Purification and E7 Antigen Expression Assessment.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0872-2_11
Abstract: Human papillomavirus (HPV ) has been extensively associated with the development of cervical cancer due to the expression of oncoproteins like E7. This protein can interfere with pRB tumor suppressor activity, enabling the uncontrolled proliferation… read more here.
Keywords: minicircle dna; dna; antigen expression; expression ... See more keywords
Production of Minicircle DNA Vectors Using Site-Specific Recombinases.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7169-5_20
Abstract: Minicircle DNA vectors are plasmid derivatives free of bacterial elements. These vectors are mostly provided from common plasmids via recombination by site-specific recombinases in E. coli. Absence of bacterial backbone in minicircle vectors results in… read more here.
Keywords: minicircle dna; site specific; minicircle; specific recombinases ... See more keywords
Highly selective capture of minicircle DNA biopharmaceuticals by a novel zinc-histidine peptide conjugate
Sign Up to like & getrecommendations! Published in 2017 at "Separation and Purification Technology"
DOI: 10.1016/j.seppur.2016.10.054
Abstract: Abstract The use of minicircle DNA (mcDNA) biomolecules as a pharmaceutical product holds remarkable potential due to their improved therapeutic efficacy in comparison with standard non-viral gene expression vectors. However, mcDNA translation into clinical application… read more here.
Keywords: histidine; minicircle dna; novel zinc; dna biopharmaceuticals ... See more keywords
Minicircle DNA: The Future for DNA-Based Vectors?
Sign Up to like & getrecommendations! Published in 2020 at "Trends in biotechnology"
DOI: 10.1016/j.tibtech.2020.04.008
Abstract: Minicircle DNA (mcDNA) is a smaller and safer version of non-viral DNA vectors that results from a cutting-edge in vivo recombination process to excise prokaryotic sequences from plasmid DNA (pDNA). Considering the molecule's potential and… read more here.
Keywords: minicircle dna; dna based; dna; based vectors ... See more keywords
Minicircle DNA-Engineered CAR T Cells Suppressed Tumor Growth in Mice
Sign Up to like & getrecommendations! Published in 2019 at "Molecular Cancer Therapeutics"
DOI: 10.1158/1535-7163.mct-19-0204
Abstract: Viral-based chimeric antigen receptor-engineered T (CAR T)–cell manufacturing has potential safety risks and relatively high costs. The nonviral minicircle DNA (mcDNA) is safer for patients, cheaper to produce, and may be a more suitable technique… read more here.
Keywords: car cells; dna engineered; minicircle dna; engineered car ... See more keywords
Synthesis and Characterization of Mannosylated Formulations to Deliver a Minicircle DNA Vaccine
Sign Up to like & getrecommendations! Published in 2021 at "Pharmaceutics"
DOI: 10.3390/pharmaceutics13050673
Abstract: DNA vaccines still represent an emergent area of research, giving rise to continuous progress towards several biomedicine demands. The formulation of delivery systems to specifically target mannose receptors, which are overexpressed on antigen presenting cells… read more here.
Keywords: minicircle dna; dna vaccine; deliver minicircle; dna ... See more keywords