Increasing the buffering capacity of minimal media leads to higher protein yield
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Biomolecular NMR"
DOI: 10.1007/s10858-018-00222-4
Abstract: We describe a general and simple modification to the standard M9 minimal medium recipe that leads to an approximate twofold increase in the yield of heterologously expressed proteins in Escherichia coli BL21(DE3) bacteria. We monitored… read more here.
Keywords: capacity minimal; protein yield; increasing buffering; yield ... See more keywords
Effects of minimal media vs. complex media on the metabolite profiles of Escherichia coli and Saccharomyces cerevisiae
Sign Up to like & getrecommendations! Published in 2017 at "Process Biochemistry"
DOI: 10.1016/j.procbio.2017.04.003
Abstract: Abstract Culture media are important environmental factors in microbial growth, and strongly affect various cellular metabolic processes. This study used metabolomics to investigate the effect of minimal and complex media on the metabolic profiles of… read more here.
Keywords: saccharomyces cerevisiae; profiles escherichia; escherichia coli; complex media ... See more keywords
A screening system using minimal media identifies a flavin-competing inhibitor of Porphyromonas gingivalis growth.
Sign Up to like & getrecommendations! Published in 2019 at "FEMS microbiology letters"
DOI: 10.1093/femsle/fnz204
Abstract: Chronic periodontitis is caused by dysbiosis of human oral commensals, and especially by increases in Porphyromonas gingivalis. Inhibitors of P. gingivalis growth are expected to serve as effective drugs for curing this disease. In the… read more here.
Keywords: gingivalis growth; porphyromonas gingivalis; gingivalis; using minimal ... See more keywords