Rapid bacterial identification by direct PCR amplification of 16S rRNA genes using the MinION™ nanopore sequencer
Sign Up to like & getrecommendations! Published in 2019 at "FEBS Open Bio"
DOI: 10.1002/2211-5463.12590
Abstract: Rapid identification of bacterial pathogens is crucial for appropriate and adequate antibiotic treatment, which significantly improves patient outcomes. 16S ribosomal RNA (rRNA) gene amplicon sequencing has proven to be a powerful strategy for diagnosing bacterial… read more here.
Keywords: identification; 16s rrna; minion; direct pcr ... See more keywords
Serogroup-level resolution of the “Super-7” Shiga toxin-producing Escherichia coli using nanopore single-molecule DNA sequencing
Sign Up to like & getrecommendations! Published in 2018 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-018-0877-1
Abstract: DNA sequencing and other DNA-based methods are now broadly used for detection and identification of bacterial foodborne pathogens. For the identification of foodborne bacterial pathogens, taxonomic assignments must be made to the species or even… read more here.
Keywords: super shiga; single molecule; dna sequencing; dna ... See more keywords
Forensic nanopore sequencing of STRs and SNPs using Verogen’s ForenSeq DNA Signature Prep Kit and MinION
Sign Up to like & getrecommendations! Published in 2021 at "International Journal of Legal Medicine"
DOI: 10.1007/s00414-021-02604-0
Abstract: The MinION nanopore sequencing device (Oxford Nanopore Technologies, Oxford, UK) is the smallest commercially available sequencer and can be used outside of conventional laboratories. The use of the MinION for forensic applications, however, is hindered… read more here.
Keywords: using verogen; forenseq dna; strs; minion ... See more keywords
Revealing antimicrobial resistance in stormwater with MinION
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DOI: 10.1016/j.chemosphere.2020.127392
Abstract: Abstract Discharge of urban stormwater containing organic matter, heavy metals and sometime human feces, to the natural aquatic reservoirs without any treatment is not only an environmental problem. It can lead to prevalence of antibiotic… read more here.
Keywords: revealing antimicrobial; resistance stormwater; minion; resistance ... See more keywords
Simultaneous amplicon analysis of multiple soil samples using MinION sequencing.
Sign Up to like & getrecommendations! Published in 2021 at "MethodsX"
DOI: 10.1016/j.mex.2021.101576
Abstract: The diversity and composition of soil microorganisms needs to be understood as they influence ecosystem processes. MinION is a relatively recent next-generation sequencer, which provides the advantage of sequencing long reads. In this study, two… read more here.
Keywords: minion sequencing; soil; minion; using minion ... See more keywords
Highly accurate-single chromosomal complete genomes using IonTorrent and MinION sequencing of clinical pathogens.
Sign Up to like & getrecommendations! Published in 2019 at "Genomics"
DOI: 10.1016/j.ygeno.2019.04.006
Abstract: Oxford Nanopore MinION sequencing technology has been gaining immense importance in identification of pathogen and antimicrobial resistance, though with 10-15% error rate. Short read technologies generates high accurate genome but with multiple fragments of genome.… read more here.
Keywords: iontorrent minion; highly accurate; single chromosomal; minion ... See more keywords
The microprotein Minion controls cell fusion and muscle formation
Sign Up to like & getrecommendations! Published in 2017 at "Nature Communications"
DOI: 10.1038/ncomms15664
Abstract: Although recent evidence has pointed to the existence of small open reading frame (smORF)-encoded microproteins in mammals, their function remains to be determined. Skeletal muscle development requires fusion of mononuclear progenitors to form multinucleated myotubes,… read more here.
Keywords: cell fusion; muscle; minion; microprotein minion ... See more keywords
Low-cost assembly of a cacao crop genome is able to resolve complex heterozygous bubbles
Sign Up to like & getrecommendations! Published in 2019 at "Horticulture Research"
DOI: 10.1038/s41438-019-0125-7
Abstract: Cacao (Theobroma cacao) is a tropical tree that produces the essential raw material for chocolate. Because yields have been stagnant, land use has expanded to provide for increasing chocolate demand. Assembled genomes of key parents… read more here.
Keywords: low cost; assembly; minion; genome ... See more keywords
Systematic and stochastic influences on the performance of the MinION nanopore sequencer across a range of nucleotide bias
Sign Up to like & getrecommendations! Published in 2018 at "Scientific Reports"
DOI: 10.1038/s41598-018-21484-w
Abstract: Emerging sequencing technologies are allowing us to characterize environmental, clinical and laboratory samples with increasing speed and detail, including real-time analysis and interpretation of data. One example of this is being able to rapidly and… read more here.
Keywords: range; minion nanopore; minion; performance ... See more keywords
Diagnosis and characterization of canine distemper virus through sequencing by MinION nanopore technology
Sign Up to like & getrecommendations! Published in 2019 at "Scientific Reports"
DOI: 10.1038/s41598-018-37497-4
Abstract: Prompt identification of the causative pathogen of an infectious disease is essential for the choice of treatment or preventive measures. In this perspective, nucleic acids purified from the brain tissue of a dog succumbed after… read more here.
Keywords: distemper virus; minion; canine distemper; technology ... See more keywords
De novo Assembly of the Brugia malayi Genome Using Long Reads from a Single MinION Flowcell
Sign Up to like & getrecommendations! Published in 2019 at "Scientific Reports"
DOI: 10.1038/s41598-019-55908-y
Abstract: Filarial nematode infections cause a substantial global disease burden. Genomic studies of filarial worms can improve our understanding of their biology and epidemiology. However, genomic information from field isolates is limited and available reference genomes… read more here.
Keywords: minion flowcell; brugia malayi; minion; single minion ... See more keywords