Development of circulating microRNA-based biomarkers for medical decision-making: a friendly reminder of what should NOT be done
Sign Up to like & getrecommendations! Published in 2022 at "Critical Reviews in Clinical Laboratory Sciences"
DOI: 10.1080/10408363.2022.2128030
Abstract: Abstract Circulating cell-free microRNAs (miRNAs) represent a major reservoir for biomarker discovery. Unfortunately, their implementation in clinical practice is limited due to a profound lack of reproducibility. The great technical variability linked to major pre-analytical… read more here.
Keywords: circulating microrna; development circulating; microrna based; mirna quantification ... See more keywords
OUHP: an optimized universal hairpin primer system for cost-effective and high-throughput RT-qPCR-based quantification of microRNA (miRNA) expression
Sign Up to like & getrecommendations! Published in 2021 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkab1153
Abstract: Abstract MicroRNAs (miRNAs or miRs) are single-stranded, ∼22-nucleotide noncoding RNAs that regulate many cellular processes. While numerous miRNA quantification technologies are available, a recent analysis of 12 commercial platforms revealed high variations in reproducibility, sensitivity,… read more here.
Keywords: mirna quantification; system; quantification; universal hairpin ... See more keywords