Pulsed SILAC as a Approach for miRNA Targets Identification in Cell Culture.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6730-8_11
Abstract: Pulsed stable isotope labeling by amino acids in cell culture (pSILAC) comprises a variation of the classical SILAC proteomic methodology that enables the identification of short-term proteomic responses such as those elicited by micro RNAs… read more here.
Keywords: approach mirna; silac approach; cell culture; mirna targets ... See more keywords
Identifying miRNA Targets Using AGO-RIPseq.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7540-2_9
Abstract: microRNAs (miRNA) are small, noncoding RNAs that bind to messenger RNAs (mRNAs) and regulate their activity. They are, therefore, important posttranscriptional regulators. In recent years it has become clear that miRNAs regulate large genetic networks,… read more here.
Keywords: targets using; using ago; ago ripseq; methodology ... See more keywords
Identification of miRNA Targets by AtFT Overexpression in Tobacco
Sign Up to like & getrecommendations! Published in 2019 at "Plant Molecular Biology Reporter"
DOI: 10.1007/s11105-019-01180-3
Abstract: The onset of flowering is regulated by complex gene networks that integrate multiple genetic cues to floral transition in plants. The highly conserved florigen FLOWERING LOCUS T (FT) functions at the core of this process.… read more here.
Keywords: tobacco; overexpression; identification mirna; mirna targets ... See more keywords
The stability and persistence of blood and semen mRNA and miRNA targets for body fluid identification in environmentally challenged and laundered samples.
Sign Up to like & getrecommendations! Published in 2019 at "Legal medicine"
DOI: 10.1016/j.legalmed.2019.03.007
Abstract: The identification of body fluids in evidentiary stains may provide investigators with probative information during an investigation. In this study, quantitative reverse transcription polymerase chain reaction (RT-qPCR) assays were performed to detect the presence of… read more here.
Keywords: blood semen; mirna targets; blood; identification ... See more keywords
High-Resolution In Vivo Identification of miRNA Targets by Halo-Enhanced Ago2 Pull-Down.
Sign Up to like & getrecommendations! Published in 2020 at "Molecular cell"
DOI: 10.1016/j.molcel.2020.05.009
Abstract: The identification of microRNA (miRNA) targets by Ago2 crosslinking-immunoprecipitation (CLIP) methods has provided major insights into the biology of this important class of non-coding RNAs. However, these methods are technically challenging and not easily applicable… read more here.
Keywords: high resolution; resolution vivo; mirna targets; ago2 ... See more keywords
High-throughput validation of ceRNA regulatory networks
Sign Up to like & getrecommendations! Published in 2017 at "BMC Genomics"
DOI: 10.1186/s12864-017-3790-7
Abstract: BackgroundMicroRNAs (miRNAs) play multiple roles in tumor biology. Interestingly, reports from multiple groups suggest that miRNA targets may be coupled through competitive stoichiometric sequestration. Specifically, computational models predicted and experimental assays confirmed that miRNA activity… read more here.
Keywords: mirna targets; cerna; throughput validation; validation cerna ... See more keywords
Prediction of miRNA targets by learning from interaction sequences
Sign Up to like & getrecommendations! Published in 2020 at "PLoS ONE"
DOI: 10.1371/journal.pone.0232578
Abstract: MicroRNAs (miRNAs) are involved in a diverse variety of biological processes through regulating the expression of target genes in the post-transcriptional level. So, it is of great importance to discover the targets of miRNAs in… read more here.
Keywords: mirna targets; target; prediction mirna; mirna target ... See more keywords
Global and precise identification of functional miRNA targets in mESCs by integrative analysis
Sign Up to like & getrecommendations! Published in 2022 at "EMBO Reports"
DOI: 10.15252/embr.202254762
Abstract: MicroRNA (miRNA) loaded Argonaute (AGO) complexes regulate gene expression via direct base pairing with their mRNA targets. Previous works suggest that up to 60% of mammalian transcripts might be subject to miRNA‐mediated regulation, but it… read more here.
Keywords: precise identification; identification functional; mirna targets; global precise ... See more keywords
Predicting miRNA targets for hepatocellular carcinoma with an integrated method
Sign Up to like & getrecommendations! Published in 2020 at "Translational cancer research"
DOI: 10.21037/tcr.2020.02.46
Abstract: MicroRNAs (miRNAs), as a kind of small non-coding RNA molecules (~22 nucleotides in length), were estimated to regulate as much as 60 % of the human protein-coding genes (1,2). miRNAs modulated the levels of post-transcriptionally… read more here.
Keywords: hepatocellular carcinoma; carcinoma integrated; mirna targets; targets hepatocellular ... See more keywords
Identification of MicroRNA Targets of Capsicum spp. Using MiRTrans—a Trans-Omics Approach
Sign Up to like & getrecommendations! Published in 2017 at "Frontiers in Plant Science"
DOI: 10.3389/fpls.2017.00495
Abstract: The microRNA (miRNA) can regulate the transcripts that are involved in eukaryotic cell proliferation, differentiation, and metabolism. Especially for plants, our understanding of miRNA targets, is still limited. Early attempts of prediction on sequence alignments… read more here.
Keywords: mirna; targets capsicum; mirna transcript; mirna targets ... See more keywords
Detecting significant features in modeling microRNA-target interactions
Sign Up to like & getrecommendations! Published in 2017 at "PeerJ"
DOI: 10.7287/peerj.preprints.3337v1
Abstract: Istituto di Biomedicina ed Immunologia Molecolare (IBIM) CNR, Palermo, Italy;; Fondazione Ri.MED, Palermo Italy;; Dipartimento di scienze e Tecnologie Biologiche Chimiche e Farmaceutiche, Università degli Studi di Palermo, Italy Corresponding author: Claudia Coronnello email: [email protected]… read more here.
Keywords: features modeling; detecting significant; mirna targets; target ... See more keywords