Profiling single-guide RNA specificity reveals a mismatch sensitive core sequence
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DOI: 10.1038/srep40638
Abstract: Targeting specificity is an essential issue in the development of CRISPR-Cas technology. Using a luciferase activation assay, off-target cleavage activity of sgRNA was systematically investigated on single nucleotide-mismatched targets. In addition to confirming that PAM-proximal… read more here.
Keywords: sensitive core; mismatch sensitive; core sequence; sequence ... See more keywords