Comparison of mobility shift affinity capillary electrophoresis and capillary electrophoresis frontal analysis for binding constant determination between human serum albumin and small drugs
Sign Up to like & getrecommendations! Published in 2022 at "ELECTROPHORESIS"
DOI: 10.1002/elps.202100320
Abstract: In this study, two capillary electrophoresis–based ligand binding assays, namely, mobility shift affinity capillary electrophoresis (ms‐ACE) and capillary electrophoresis‐frontal analysis (CE‐FA), were applied to determine binding parameters of human serum albumin toward small drugs under… read more here.
Keywords: affinity capillary; shift affinity; capillary electrophoresis; electrophoresis frontal ... See more keywords
Detection of DNA Autoantibodies by Electrophoretic Mobility Shift Assay.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8949-2_11
Abstract: Although the presence of antibodies against double-stranded (ds) DNA is considered the serological hallmark of systemic lupus erythematosus (SLE), it is not detected in all SLE patients by routine laboratory tests. Looking at DNA-anti(ds)DNA interaction… read more here.
Keywords: shift assay; mobility shift; electrophoretic mobility; dna ... See more keywords
An electrophoretic mobility shift assay using the protein isolated from host plants
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DOI: 10.1186/s13007-024-01201-7
Abstract: The electrophoretic mobility shift assay (EMSA) is a common technology to detect DNA-protein interactions. However, in most cases, the protein used in EMSA is obtained via prokaryotic expression, and rarely from plants. At the same… read more here.
Keywords: host; shift; electrophoretic mobility; protein ... See more keywords
An electrophoretic mobility shift assay with chemiluminescent readout to evaluate DNA-targeting oligonucleotide-based probes
Sign Up to like & getrecommendations! Published in 2025 at "PLOS One"
DOI: 10.1371/journal.pone.0335674
Abstract: A comprehensive and user-friendly method for evaluating recognition of double-stranded DNA (dsDNA) targets by oligonucleotide-based probes is presented. Thus, dsDNA-targeting probes such as single-stranded locked nucleic acids (LNAs) and double-stranded Invader probes are incubated with… read more here.
Keywords: electrophoretic mobility; oligonucleotide based; shift assay; mobility shift ... See more keywords