COI metabarcoding of large benthic Foraminifera: Method validation for application in ecological studies
Sign Up to like & getrecommendations! Published in 2022 at "Ecology and Evolution"
DOI: 10.1002/ece3.9549
Abstract: Abstract Monitoring community composition of Foraminifera (single‐celled marine protists) provides valuable insights into environmental conditions in marine ecosystems. Despite the efficiency of environmental DNA (eDNA) and bulk‐sample DNA (bulk‐DNA) metabarcoding to assess the presence of… read more here.
Keywords: dna; large benthic; bulk dna; foraminifera ... See more keywords
Using mock communities of arbuscular mycorrhizal fungi to evaluate fidelity associated with Illumina sequencing
Sign Up to like & getrecommendations! Published in 2018 at "Fungal Ecology"
DOI: 10.1016/j.funeco.2018.01.004
Abstract: Abstract Characterization of arbuscular mycorrhizal (AM) fungal communities increasingly relies on high throughput sequencing (HTS) datasets, but whether sequence data accurately depict AM fungal communities is unknown. We sequenced mock communities of 16 AM fungal… read more here.
Keywords: using mock; communities arbuscular; relative abundance; mock communities ... See more keywords
Comprehensive single-PCR 16S and 18S rRNA community analysis validated with mock communities, and estimation of sequencing bias against 18S.
Sign Up to like & getrecommendations! Published in 2021 at "Environmental microbiology"
DOI: 10.1111/1462-2920.15553
Abstract: Universal primers for SSU rRNA genes allow profiling of natural communities by simultaneously amplifying templates from Bacteria, Archaea, and Eukaryota in a single PCR reaction. Despite the potential to show relative abundance for all rRNA… read more here.
Keywords: rrna; mock; universal primers; pcr ... See more keywords
MinION Sequencing of Yeast Mock Communities To Assess the Effect of Databases and ITS-LSU Markers on the Reliability of Metabarcoding Analysis
Sign Up to like & getrecommendations! Published in 2022 at "Microbiology Spectrum"
DOI: 10.1128/spectrum.01052-22
Abstract: Metagenomic analyses are fundamental in many research areas; therefore, improvement of methods and protocols for the description of microbial communities becomes more and more necessary. Long-read sequencing could be used for reducing biases due to… read more here.
Keywords: effect; identification; mock; minion ... See more keywords
Characterization and Demonstration of Mock Communities as Control Reagents for Accurate Human Microbiome Community Measurements
Sign Up to like & getrecommendations! Published in 2022 at "Microbiology Spectrum"
DOI: 10.1128/spectrum.01915-21
Abstract: Application of high-throughput DNA sequencing has greatly accelerated human microbiome research and its translation into new therapeutic and diagnostics capabilities. Microbiome community analyses results can, however, vary considerably across studies or laboratories, and establishment of… read more here.
Keywords: microbiome community; mock communities; human microbiome; mock ... See more keywords