Ultrasensitive Detection of m6 A-Modified RNA Using CRISPR/Cas12a-Integrated Iontronic Biosensor with Hydrophobized Nanochannels: Toward Early Cancer Diagnosis by Machine Learning.
Sign Up to like & getrecommendations! Published in 2025 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.5c05377
Abstract: N6 -methyladenosine (m6 A), the most prevalent internal modification in eukaryotic RNAs, has emerged as a focal point of intensive research in recent years owing to its pivotal regulatory roles in carcinogenesis, progression, and metastasis.… read more here.
Keywords: detection; cancer; iontronic biosensor; crispr cas12a ... See more keywords
Selective Inhibition of ADAR1 Using 8-Azanebularine-Modified RNA Duplexes.
Sign Up to like & getrecommendations! Published in 2023 at "Biochemistry"
DOI: 10.1021/acs.biochem.2c00686
Abstract: Adenosine deaminases acting on RNA (ADARs) are RNA editing enzymes that catalyze the hydrolytic deamination of adenosine (A) to inosine (I) in dsRNA. In humans, two catalytically active ADARs, ADAR1 and ADAR2, perform this A-to-I… read more here.
Keywords: modified rna; selective inhibition; rna; rna duplexes ... See more keywords
Electrochemical Biosensor Using DNA Embedded Phosphorothioate Modified RNA for Mercury Ion Determination.
Sign Up to like & getrecommendations! Published in 2018 at "ACS sensors"
DOI: 10.1021/acssensors.7b00892
Abstract: Mercury (Hg) and its compounds, originating from a variety of natural and anthropogenic sources, are ubiquitous in the natural environment, and cause severe environmental contamination and pose irreversible harm to human health. A fast and… read more here.
Keywords: biosensor using; modified rna; dna embedded; mercury ... See more keywords
SARS-CoV-2 Nsp1-Resistant Modified RNA for the Creation of Nsp1-Responsive Systems.
Sign Up to like & getrecommendations! Published in 2025 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.5c00075
Abstract: Modified RNA (modRNA) facilitates the introduction of complex synthetic genetic circuits into cells without the risk of genomic integration, opening up the implementation of synthetic circuits as therapeutics. However, the number of protein-RNA interfaces that… read more here.
Keywords: sars cov; cov nsp1; modified rna; creation ... See more keywords
Towards a comprehensive understanding of RNA deamination: synthesis and properties of xanthosine-modified RNA
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac477
Abstract: Abstract Nucleobase deamination, such as A-to-I editing, represents an important posttranscriptional modification of RNA. When deamination affects guanosines, a xanthosine (X) containing RNA is generated. However, the biological significance and chemical consequences on RNA are… read more here.
Keywords: modified rna; deamination; towards comprehensive; rna deamination ... See more keywords
Bioorthogonal labeling and profiling of N6-isopentenyladenosine (i6A) modified RNA
Sign Up to like & getrecommendations! Published in 2024 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkae150
Abstract: Abstract Chemical modifications in RNAs play crucial roles in diversifying their structures and regulating numerous biochemical processes. Since the 1990s, several hydrophobic prenyl-modifications have been discovered in various RNAs. Prenyl groups serve as precursors for… read more here.
Keywords: bioorthogonal labeling; modified rna; isopentenyladenosine i6a; labeling profiling ... See more keywords
Single-cell imaging of N4-acetylcytidine-modified RNA using fluorine metabolic labeling mediated proximity ligation assay
Sign Up to like & getrecommendations! Published in 2025 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkaf464
Abstract: Abstract N 4-Acetylcytidine (ac4C) is an emerging epitranscriptomic mark involved in regulating RNA stability, translation, and gene expression. Despite its emerging role in gene regulation and disease, current methods for in situ detection of ac4C-modified… read more here.
Keywords: ligation assay; metabolic labeling; modified rna; proximity ligation ... See more keywords
Enzymatic Conjugation of Modified RNA Fragments by Ancestral RNA Ligase AncT4_2
Sign Up to like & getrecommendations! Published in 2022 at "Applied and Environmental Microbiology"
DOI: 10.1128/aem.01679-22
Abstract: RNA therapeutics is one of the next-generation medicines for treating various diseases. Our designed ancestral RNA ligase AncT4_2 exhibited excellent enzymatic properties, such as high thermal stability, productivity, specific activity, and broad substrate selectivity compared… read more here.
Keywords: modified rna; rna; anct4; rna fragments ... See more keywords
PNPase and RhlB Interact and Reduce the Cellular Availability of Oxidized RNA in Deinococcus radiodurans
Sign Up to like & getrecommendations! Published in 2022 at "Microbiology Spectrum"
DOI: 10.1128/spectrum.02140-22
Abstract: Oxidative RNA damage can be caused by oxidative stress, such as hydrogen peroxide, ionizing radiation, and antibiotic treatment. 8-oxo-7,8-dihydroguanine (8-oxoG), a major type of oxidized RNA, is highly mutagenic and participates in a variety of… read more here.
Keywords: modified rna; oxidized rna; rna; oxidative stress ... See more keywords
Challenges with Simulating Modified RNA: Insights into Role and Reciprocity of Experimental and Computational Approaches
Sign Up to like & getrecommendations! Published in 2022 at "Genes"
DOI: 10.3390/genes13030540
Abstract: RNA is critical to a broad spectrum of biological and viral processes. This functional diversity is a result of their dynamic nature; the variety of three-dimensional structures that they can fold into; and a host… read more here.
Keywords: rna insights; challenges simulating; role; simulating modified ... See more keywords
2′-Fluoro-Pyrimidine-Modified RNA Aptamers Specific for Lipopolysaccharide Binding Protein (LBP)
Sign Up to like & getrecommendations! Published in 2018 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms19123883
Abstract: Lipopolysaccaride binding protein (LBP), a glycosylated acute phase protein, plays an important role in the pathophysiology of sepsis. LBP binds with high affinity to the lipid part of bacterial lipopolysaccaride (LPS). Inhibition of the LPS-LBP… read more here.
Keywords: protein; aptamers specific; lbp; protein lbp ... See more keywords