Integrated Strategies for Enhancing the Expression of the AqCoA Chitosanase in Pichia pastoris by Combined Optimization of Molecular Chaperones Combinations and Copy Numbers via a Novel Plasmid pMC-GAP
Sign Up to like & getrecommendations! Published in 2021 at "Applied Biochemistry and Biotechnology"
DOI: 10.1007/s12010-021-03668-9
Abstract: In our previous study, the chitosanase AqCoA and the chitooligosaccharides it produced were found to exhibit significant protective effects against fungal diseases. In this study, we enhanced the expression of AqCoA using the novel pMC-GAP… read more here.
Keywords: gap; expression; aqcoa; expression aqcoa ... See more keywords
Channeling Nascent Proteins Towards the Native State: Role of the Ribosome and Molecular Chaperones
Sign Up to like & getrecommendations! Published in 2017 at "Biophysical Journal"
DOI: 10.1016/j.bpj.2016.11.113
Abstract: Despite much progress over the last six decades, we are still far from understanding the mechanism of protein folding and aggregation in the cell. This lack of information poses tremendous challenges to progress in many… read more here.
Keywords: nascent proteins; ribosome molecular; protein folding; role ... See more keywords
Efficient production of aggregation prone 4-α-glucanotransferase by combined use of molecular chaperones and chemical chaperones in Escherichia coli.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of biotechnology"
DOI: 10.1016/j.jbiotec.2019.01.014
Abstract: In this study, a combined optimization strategy, based on co-expression of molecular chaperones and supplementation of osmolytes, was used to reduce the formation of inclusion bodies and enhance the expression of the soluble form of… read more here.
Keywords: aggregation prone; production; molecular chaperones; cell ... See more keywords
Targeting metabolic syndrome with phytochemicals: Focus on the role of molecular chaperones and hormesis in drug discovery.
Sign Up to like & getrecommendations! Published in 2020 at "Pharmacological research"
DOI: 10.1016/j.phrs.2020.104925
Abstract: Adaptive cellular stress response confers stress tolerance against inflammatory and metabolic disorders. In response to metabolic stress, the key mediator of cellular adaptation and tolerance is a class of molecules called the molecular chaperones (MCs).… read more here.
Keywords: drug discovery; role; metabolic syndrome; molecular chaperones ... See more keywords
Expression and characterisation of human glycerol kinase: the role of solubilising agents and molecular chaperones
Sign Up to like & getrecommendations! Published in 2023 at "Bioscience Reports"
DOI: 10.1042/bsr20222258
Abstract: Abstract Background: Glycerol kinase (GK; EC 2.7.1.30) facilitates the entry of glycerol into pathways of glucose and triglyceride metabolism and may play a potential role in Type 2 diabetes mellitus (T2DM). However, the detailed regulatory… read more here.
Keywords: molecular chaperones; expression; glycerol; glycerol kinase ... See more keywords
Copper Induces Protein Aggregation, a Toxic Process Compensated by Molecular Chaperones
Sign Up to like & getrecommendations! Published in 2022 at "mBio"
DOI: 10.1128/mbio.03251-21
Abstract: With the increase of antibiotic drug resistance, alternative antibacterial treatment strategies are needed. Copper is a well-known antimicrobial and antiviral agent; however, the underlying molecular mechanisms by which copper causes cell death are not yet… read more here.
Keywords: molecular chaperones; copper; toxicity; protein aggregation ... See more keywords
Role of Mitochondrial Heat-shock Proteins and Immunophilins in Neuro degenerative Diseases.
Sign Up to like & getrecommendations! Published in 2020 at "Current drug targets"
DOI: 10.2174/1389450121999201230204320
Abstract: Pathophysiologic conditions of neurodegenerative diseases are unquestionably related to protein misfolding. The accumulation of misfolded proteins into relatively ordered structures such as fibrillar intracellular and extracellular amyloids results in tissue lesions that lead to neuronal… read more here.
Keywords: heat shock; shock proteins; molecular chaperones; proteins immunophilins ... See more keywords
Plasmodium falciparum Molecular Chaperones: Guardians of the Malaria Parasite Proteome and Renovators of the Host Proteome
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Cell and Developmental Biology"
DOI: 10.3389/fcell.2022.921739
Abstract: Plasmodium falciparum is a unicellular protozoan parasite and causative agent of the most severe form of malaria in humans. The malaria parasite has had to develop sophisticated mechanisms to preserve its proteome under the changing… read more here.
Keywords: plasmodium falciparum; molecular chaperones; malaria parasite; falciparum molecular ... See more keywords
Bacillus subtilis forms twisted cells with cell wall integrity defects upon removal of the molecular chaperones DnaK and trigger factor
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2022.988768
Abstract: The protein homeostasis network ensures a proper balance between synthesis, folding, and degradation of all cellular proteins. DnaK and trigger factor (TF) are ubiquitous bacterial molecular chaperones that assist in protein folding, as well as… read more here.
Keywords: molecular chaperones; cell wall; dnak trigger; wall integrity ... See more keywords
Neurotransmitters and molecular chaperones interactions in cerebral malaria: Is there a missing link?
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Molecular Biosciences"
DOI: 10.3389/fmolb.2022.965569
Abstract: Plasmodium falciparum is responsible for the most severe and deadliest human malaria infection. The most serious complication of this infection is cerebral malaria. Among the proposed hypotheses that seek to explain the manifestation of the… read more here.
Keywords: molecular chaperones; pathology; falciparum; cerebral malaria ... See more keywords
Myelin Pathology: Involvement of Molecular Chaperones and the Promise of Chaperonotherapy
Sign Up to like & getrecommendations! Published in 2019 at "Brain Sciences"
DOI: 10.3390/brainsci9110297
Abstract: The process of axon myelination involves various proteins including molecular chaperones. Myelin alteration is a common feature in neurological diseases due to structural and functional abnormalities of one or more myelin proteins. Genetic proteinopathies may… read more here.
Keywords: involvement molecular; pathology; pathology involvement; molecular chaperones ... See more keywords