Molecular cloning and characterization of five genes from embryogenic callus in Miscanthus lutarioriparius
Sign Up to like & getrecommendations! Published in 2020 at "Acta Physiologiae Plantarum"
DOI: 10.1007/s11738-020-03071-7
Abstract: The regeneration from embryogenic callus of higher plants in tissue culture is regulated by explants types and developmental stage and also regulated by some genes. In Miscanthus lutarioriparius, five candidate genes were selected to decide… read more here.
Keywords: molecular cloning; cloning characterization; callus; miscanthus lutarioriparius ... See more keywords
Molecular cloning and functional characterization of a sweetpotato chloroplast IbDHAR3 gene in response to abiotic stress
Sign Up to like & getrecommendations! Published in 2019 at "Plant Biotechnology Reports"
DOI: 10.1007/s11816-019-00576-7
Abstract: Dehydroascorbate reductase (DHAR) plays a critical role in the regeneration of ascorbic acid (AsA), and widely involved in plant tolerance to biotic and abiotic stresses. In this study, the IbDHAR3 gene was cloned from sweetpotato… read more here.
Keywords: molecular cloning; functional characterization; chloroplast; cloning functional ... See more keywords
Molecular cloning and expression analysis of GT-2-like genes in strawberry
Sign Up to like & getrecommendations! Published in 2019 at "3 Biotech"
DOI: 10.1007/s13205-019-1603-1
Abstract: GT-2 factors are the members of trihelix transcription factors (TFs) which can function in regulating plant development and responding to different abiotic stress. These proteins contain two structural domains composed by three tandem repeats helix–loop–helix–loop–helix.… read more here.
Keywords: molecular cloning; fagt like; analysis; expression ... See more keywords
Skin secretion transcriptome remains in chromatographic fractions suitable for molecular cloning.
Sign Up to like & getrecommendations! Published in 2019 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2018.10.006
Abstract: Traditional sources of mRNA for molecular cloning on amphibian skin secretion studies have been the frog's skin and skin secretions. Here, we demonstrate that mRNA isolated from chromatographic fractions of skin secretions is amenable for… read more here.
Keywords: molecular cloning; chromatographic fractions; transcriptome remains; secretion transcriptome ... See more keywords
Molecular cloning and immune function study of an oyster IκB gene in the NF-κB signaling pathway
Sign Up to like & getrecommendations! Published in 2020 at "Aquaculture"
DOI: 10.1016/j.aquaculture.2020.735322
Abstract: Abstract Mass mortality caused by pathogens has severely affected the oyster cultivation industry, but our knowledge about the innate immune system of oysters is still limited. The nuclear factor-κB (NF-κB) signaling pathway plays a vital… read more here.
Keywords: molecular cloning; signaling pathway; function; gene ... See more keywords
Identification and molecular cloning of novel transcripts of the human kallikrein-related peptidase 10 (KLK10) gene using next-generation sequencing.
Sign Up to like & getrecommendations! Published in 2017 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2017.04.078
Abstract: Tissue kallikrein and kallikrein-related peptidases (KLKs) form the largest group of serine proteases in the human genome, sharing many structural and functional characteristics. Multiple alternative transcripts have been reported for the most human KLK genes,… read more here.
Keywords: molecular cloning; identification molecular; klk10; kallikrein related ... See more keywords
Molecular cloning and functional characterization of duck DDX41
Sign Up to like & getrecommendations! Published in 2018 at "Developmental and Comparative Immunology"
DOI: 10.1016/j.dci.2018.07.015
Abstract: ABSTRACT DEAD (Asp–Glu–Ala–Asp) box polypeptide 41 (DDX41), a receptor belonging to DExD/H‐box helicase family, acts as an intracellular DNA sensor and induces type I IFN production in mammals and fish. However, the function of avian… read more here.
Keywords: molecular cloning; ifn bgr; duck ddx41; duck ... See more keywords
Molecular cloning and functional characterization of elongase (elovl5) and fatty acyl desaturase (fads2) in sciaenid, Nibea diacanthus (Lacepède, 1802).
Sign Up to like & getrecommendations! Published in 2019 at "Gene"
DOI: 10.1016/j.gene.2019.01.033
Abstract: In the present paper, we investigated the molecular cloning and functional characterization of elongase of very long chain fatty acid (elovl) and fatty acyl desaturase (fads) genes in a marine teleost, Nibea diacanthus. The elongase… read more here.
Keywords: molecular cloning; desaturase; elongase; functional characterization ... See more keywords
Molecular cloning and biochemical characterization of a trehalose synthase from Myxococcus sp. strain V11.
Sign Up to like & getrecommendations! Published in 2021 at "Protein expression and purification"
DOI: 10.1016/j.pep.2021.105865
Abstract: The tresI gene of Myxococcus sp. strain V11 was cloned, and found to encode a trehalose synthase comprising 551 amino acids. The deduced molecular weight of the encoded TreS I protein 64.7 kDa and the… read more here.
Keywords: trehalose synthase; molecular cloning; myxococcus strain; strain v11 ... See more keywords
Molecular cloning and functional characterization of an isoflavone glucosyltransferase from Pueraria thomsonii.
Sign Up to like & getrecommendations! Published in 2022 at "Chinese journal of natural medicines"
DOI: 10.1016/s1875-5364(21)60105-x
Abstract: Pueraria thomsonii has long been used in traditional Chinese medicine. Isoflavonoids are the principle pharmacologically active components, which are primarily observed as glycosyl-conjugates and accumulate in P. thomsonii roots. However, the molecular mechanisms underlying the… read more here.
Keywords: glucosyltransferase; pueraria thomsonii; cloning functional; functional characterization ... See more keywords
Molecular cloning, tissue distribution and function analysis of duck TLR7.
Sign Up to like & getrecommendations! Published in 2020 at "Animal biotechnology"
DOI: 10.1080/10495398.2020.1784186
Abstract: Toll-like receptors (TLRs) play an important role in detecting pathogen-associated molecular patterns (PAMPs). Among the TLRs, TLR7 is involved in the recognition of antiviral compounds and single-stranded RNA. This study was designed to explore the… read more here.
Keywords: molecular cloning; tlr7; duck tlr7; duck ... See more keywords