Insufficiently complex unique-molecular identifiers (UMIs) distort small RNA sequencing
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-71323-0
Abstract: The attachment of unique molecular identifiers (UMIs) to RNA molecules prior to PCR amplification and sequencing, makes it possible to amplify libraries to a level that is sufficient to identify rare molecules, whilst simultaneously eliminating… read more here.
Keywords: small rna; identifiers umis; rna; insufficiently complex ... See more keywords
Accurate estimation of molecular counts from amplicon sequence data with unique molecular identifiers
Sign Up to like & getrecommendations! Published in 2022 at "Bioinformatics"
DOI: 10.1101/2022.06.12.495839
Abstract: Motivation Amplicon sequencing is widely applied to explore heterogeneity and rare variants in genetic populations. Resolving true biological variants and quantifying their abundance is crucial for downstream analyses, but measured abundances are distorted by stochasticity… read more here.
Keywords: abundance; amplicon sequence; estimation; sequence data ... See more keywords
Targeted RNAseq assay incorporating unique molecular identifiers for improved quantification of gene expression signatures and transcribed mutation fraction in fixed tumor samples
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DOI: 10.1186/s12885-021-07814-8
Abstract: Background Our objective was to assess whether modifications to a customized targeted RNA sequencing (RNAseq) assay to include unique molecular identifiers (UMIs) that collapse read counts to their source mRNA counts would improve quantification of… read more here.
Keywords: ffpe samples; set4 assay; rnaseq assay; unique molecular ... See more keywords
Incorporation of unique molecular identifiers in TruSeq adapters improves the accuracy of quantitative sequencing.
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DOI: 10.2144/000114608
Abstract: Quantitative analysis of next-generation sequencing (NGS) data requires discriminating duplicate reads generated by PCR from identical molecules that are of unique origin. Typically, PCR duplicates are identified as sequence reads that align to the same… read more here.
Keywords: sequencing incorporation; molecular identifiers; unique molecular; incorporation unique ... See more keywords