On the interpretation of kinetics and thermodynamics probed by single-molecule experiments
Sign Up to like & getrecommendations! Published in 2020 at "Colloid and Polymer Science"
DOI: 10.1007/s00396-020-04662-z
Abstract: Single-molecule pulling experiments are widely used to extract both thermodynamic and kinetic data on ligand-receptor pairs, typically by fitting different models to the probability distribution of rupture forces of the corresponding bond. Here, a theoretical… read more here.
Keywords: single molecule; thermodynamics; bond; ligand receptor ... See more keywords
A Wi-Fi live streaming Centrifuge Force Microscope (CFM) for benchtop single-molecule experiments.
Sign Up to like & getrecommendations! Published in 2020 at "Biophysical journal"
DOI: 10.1016/j.bpj.2020.10.017
Abstract: The ability to apply controlled forces to individual molecules has been revolutionary in shaping our understanding of biophysics in areas as diverse as dynamic bond strength, biological motor operation, and DNA replication. However, the methodology… read more here.
Keywords: single molecule; microscope; cfm; force ... See more keywords
Loop extrusion: theory meets single-molecule experiments.
Sign Up to like & getrecommendations! Published in 2020 at "Current opinion in cell biology"
DOI: 10.1016/j.ceb.2020.04.011
Abstract: Chromosomes are organized as chromatin loops that promote segregation, enhancer-promoter interactions, and other genomic functions. Loops were hypothesized to form by 'loop extrusion,' by which structural maintenance of chromosomes (SMC) complexes, such as condensin and… read more here.
Keywords: single molecule; loop extrusion; extrusion theory; extrusion ... See more keywords