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   Articles with "molecule fluorescence" as a keyword



In Situ Probing of Polymer Residence on the Nanoparticle Surface by Combining Deep Learning and Single-Molecule Fluorescence Tracking.

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recommendations! Published in 2025 at "Macromolecular rapid communications"

DOI: 10.1002/marc.202500477

Abstract: This study presents a method for in situ analysis of the adsorption and desorption of polymers on the nanoparticle surface within entangled polymer solutions. This method is based on the principle that when a polymer… read more here.

Keywords: surface; nanoparticle surface; molecule fluorescence; single molecule ... See more keywords

Rapid and Sensitive Detection of miRNA by Single-Molecule Fluorescence Dequenching Assay with Target Recycled CRISPR/Cas12a Amplification System.

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recommendations! Published in 2025 at "Small"

DOI: 10.1002/smll.202412228

Abstract: Dysregulated miRNAs play a critical role in the development of cancers. A rapid and sensitive single-molecule fluorescence dequenching assay combined with a CRISPR/Cas12a-based target recycling amplification system for miRNA detection is developed. This single-molecule assay… read more here.

Keywords: single molecule; dequenching assay; molecule; rapid sensitive ... See more keywords

High-Resolution "Fleezers": Dual-Trap Optical Tweezers Combined with Single-Molecule Fluorescence Detection.

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recommendations! Published in 2017 at "Methods in molecular biology"

DOI: 10.1007/978-1-4939-6421-5_8

Abstract: Recent advances in optical tweezers have greatly expanded their measurement capabilities. A new generation of hybrid instrument that combines nanomechanical manipulation with fluorescence detection-fluorescence optical tweezers, or "fleezers"-is providing a powerful approach to study complex… read more here.

Keywords: optical tweezers; high resolution; single molecule; molecule fluorescence ... See more keywords

Single-Molecule Fluorescence Energy Transfer Assays for the Characterization of Reaction Pathways of miRNA-Argonaute Complex.

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recommendations! Published in 2017 at "Methods in molecular biology"

DOI: 10.1007/978-1-4939-6563-2_21

Abstract: Argonaute proteins are key components of the microRNA-induced silencing complexes (miRISCs) that mediate the posttranscriptional gene silencing of microRNAs and small interfering RNA (siRNAs). The complex reaction mechanism of miRISC is expected to be characterized… read more here.

Keywords: argonaute; molecule fluorescence; single molecule; reaction pathways ... See more keywords
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A Brief Introduction to Single-Molecule Fluorescence Methods.

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recommendations! Published in 2018 at "Methods in molecular biology"

DOI: 10.1007/978-1-4939-7271-5_5

Abstract: One of the more popular single-molecule approaches in biological science is single-molecule fluorescence microscopy, which will be the subject of the following section of this volume. Fluorescence methods provide the sensitivity required to study biology… read more here.

Keywords: molecule fluorescence; single molecule; biology; microscopy ... See more keywords

Single-Molecule Fluorescence Microscopy in Living Caenorhabditis elegans.

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recommendations! Published in 2018 at "Methods in molecular biology"

DOI: 10.1007/978-1-4939-7271-5_8

Abstract: Transportation of organelles and biomolecules is vital for many cellular processes. Single-molecule (SM) fluorescence microscopy can expose molecular aspects of the dynamics that remain unresolved in ensemble experiments. For example, trajectories of individual, moving biomolecules… read more here.

Keywords: molecule fluorescence; single molecule; fluorescence microscopy; microscopy ... See more keywords
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Single - Molecule Fluorescence Microscopy of Folding Dynamics of G-Quadruplex DNA

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recommendations! Published in 2017 at "Biophysical Journal"

DOI: 10.1016/j.bpj.2016.11.1184

Abstract: G-quadruplexes are unique secondary DNA structures that have been proposed to play a number of regulatory roles in cell biology [1] and are possible targets for anti-cancer therapies [2]. Here we employed single-molecule FRET microscopy… read more here.

Keywords: molecule fluorescence; single molecule; dna; microscopy ... See more keywords
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Understanding the dynamics and structure of epigenetic states with single-molecule fluorescence microscopy

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recommendations! Published in 2019 at "Current Opinion in Biomedical Engineering"

DOI: 10.1016/j.cobme.2019.08.010

Abstract: Abstract Epigenetic modifications play an important role in regulating gene expression among a host of other factors during cell development. The plasticity of epigenetic modifications has the potential to direct a cell in relation to… read more here.

Keywords: molecule fluorescence; single molecule; epigenetic states; microscopy ... See more keywords
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Small-molecule fluorescence-based probes for interrogating major organ diseases.

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recommendations! Published in 2021 at "Chemical Society reviews"

DOI: 10.1039/d0cs01183e

Abstract: Chemical tools that allow the real-time monitoring of organ function and the visualisation of organ-related processes at the cellular level are of great importance in biological research. The upregulation/downregulation of specific biomarkers is often associated… read more here.

Keywords: molecule; molecule fluorescence; small molecule; fluorescence based ... See more keywords

Simultaneous and ultrasensitive detection of multiple microRNAs by single-molecule fluorescence imaging†

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recommendations! Published in 2020 at "Chemical Science"

DOI: 10.1039/d0sc00580k

Abstract: Cell status changes are typically accompanied by the simultaneous changes of multiple microRNA (miRNA) levels. Thus, simultaneous and ultrasensitive detection of multiple miRNA biomarkers shows great promise in early cancer diagnosis. Herein, a facile single-molecule… read more here.

Keywords: detection; single molecule; molecule fluorescence; detection multiple ... See more keywords

Broadband single-molecule fluorescence enhancement based on self-assembled Ag@Au dimer plasmonic nanoantennas.

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recommendations! Published in 2022 at "Nanoscale"

DOI: 10.1039/d2nr03466b

Abstract: Dimer optical antennas (OAs) enable great fluorescence enhancement and excitation volume reduction and hence potentially can be a very useful tool for single-molecule detection. The realization of broadband fluorescence enhancement with a dimer OA remains… read more here.

Keywords: single molecule; molecule fluorescence; fluorescence enhancement; dimer ... See more keywords