High-Resolution "Fleezers": Dual-Trap Optical Tweezers Combined with Single-Molecule Fluorescence Detection.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6421-5_8
Abstract: Recent advances in optical tweezers have greatly expanded their measurement capabilities. A new generation of hybrid instrument that combines nanomechanical manipulation with fluorescence detection-fluorescence optical tweezers, or "fleezers"-is providing a powerful approach to study complex… read more here.
Keywords: optical tweezers; high resolution; single molecule; molecule fluorescence ... See more keywords
Single-Molecule Fluorescence Energy Transfer Assays for the Characterization of Reaction Pathways of miRNA-Argonaute Complex.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6563-2_21
Abstract: Argonaute proteins are key components of the microRNA-induced silencing complexes (miRISCs) that mediate the posttranscriptional gene silencing of microRNAs and small interfering RNA (siRNAs). The complex reaction mechanism of miRISC is expected to be characterized… read more here.
Keywords: argonaute; molecule fluorescence; single molecule; reaction pathways ... See more keywords
A Brief Introduction to Single-Molecule Fluorescence Methods.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7271-5_5
Abstract: One of the more popular single-molecule approaches in biological science is single-molecule fluorescence microscopy, which will be the subject of the following section of this volume. Fluorescence methods provide the sensitivity required to study biology… read more here.
Keywords: molecule fluorescence; single molecule; biology; microscopy ... See more keywords
Single-Molecule Fluorescence Microscopy in Living Caenorhabditis elegans.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7271-5_8
Abstract: Transportation of organelles and biomolecules is vital for many cellular processes. Single-molecule (SM) fluorescence microscopy can expose molecular aspects of the dynamics that remain unresolved in ensemble experiments. For example, trajectories of individual, moving biomolecules… read more here.
Keywords: molecule fluorescence; single molecule; fluorescence microscopy; microscopy ... See more keywords
Single - Molecule Fluorescence Microscopy of Folding Dynamics of G-Quadruplex DNA
Sign Up to like & getrecommendations! Published in 2017 at "Biophysical Journal"
DOI: 10.1016/j.bpj.2016.11.1184
Abstract: G-quadruplexes are unique secondary DNA structures that have been proposed to play a number of regulatory roles in cell biology [1] and are possible targets for anti-cancer therapies [2]. Here we employed single-molecule FRET microscopy… read more here.
Keywords: molecule fluorescence; single molecule; dna; microscopy ... See more keywords
Understanding the dynamics and structure of epigenetic states with single-molecule fluorescence microscopy
Sign Up to like & getrecommendations! Published in 2019 at "Current Opinion in Biomedical Engineering"
DOI: 10.1016/j.cobme.2019.08.010
Abstract: Abstract Epigenetic modifications play an important role in regulating gene expression among a host of other factors during cell development. The plasticity of epigenetic modifications has the potential to direct a cell in relation to… read more here.
Keywords: molecule fluorescence; single molecule; epigenetic states; microscopy ... See more keywords
Small-molecule fluorescence-based probes for interrogating major organ diseases.
Sign Up to like & getrecommendations! Published in 2021 at "Chemical Society reviews"
DOI: 10.1039/d0cs01183e
Abstract: Chemical tools that allow the real-time monitoring of organ function and the visualisation of organ-related processes at the cellular level are of great importance in biological research. The upregulation/downregulation of specific biomarkers is often associated… read more here.
Keywords: molecule; molecule fluorescence; small molecule; fluorescence based ... See more keywords
Simultaneous and ultrasensitive detection of multiple microRNAs by single-molecule fluorescence imagingâ€
Sign Up to like & getrecommendations! Published in 2020 at "Chemical Science"
DOI: 10.1039/d0sc00580k
Abstract: Cell status changes are typically accompanied by the simultaneous changes of multiple microRNA (miRNA) levels. Thus, simultaneous and ultrasensitive detection of multiple miRNA biomarkers shows great promise in early cancer diagnosis. Herein, a facile single-molecule… read more here.
Keywords: detection; single molecule; molecule fluorescence; detection multiple ... See more keywords
Broadband single-molecule fluorescence enhancement based on self-assembled Ag@Au dimer plasmonic nanoantennas.
Sign Up to like & getrecommendations! Published in 2022 at "Nanoscale"
DOI: 10.1039/d2nr03466b
Abstract: Dimer optical antennas (OAs) enable great fluorescence enhancement and excitation volume reduction and hence potentially can be a very useful tool for single-molecule detection. The realization of broadband fluorescence enhancement with a dimer OA remains… read more here.
Keywords: single molecule; molecule fluorescence; fluorescence enhancement; dimer ... See more keywords
Single-Molecule Fluorescence Applied to Translation.
Sign Up to like & getrecommendations! Published in 2019 at "Cold Spring Harbor perspectives in biology"
DOI: 10.1101/cshperspect.a032714
Abstract: Single-molecule fluorescence methods have illuminated the dynamics of the translational machinery. Structural and bulk biochemical experiments have provided detailed atomic and global mechanistic views of translation, respectively. Single-molecule studies of translation have bridged these views… read more here.
Keywords: molecule; molecule fluorescence; single molecule; translation ... See more keywords
Bayesian machine learning analysis of single-molecule fluorescence colocalization images.
Sign Up to like & getrecommendations! Published in 2022 at "eLife"
DOI: 10.7554/elife.73860
Abstract: Multi-wavelength single-molecule fluorescence colocalization (CoSMoS) methods allow elucidation of complex biochemical reaction mechanisms. However, analysis of CoSMoS data is intrinsically challenging because of low image signal-to-noise ratios, non-specific surface binding of the fluorescent molecules, and… read more here.
Keywords: single molecule; fluorescence colocalization; machine learning; molecule fluorescence ... See more keywords