Automation of Multiplexed RNAscope Single-Molecule Fluorescent In Situ Hybridization and Immunohistochemistry for Spatial Tissue Mapping.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0623-0_15
Abstract: In situ transcriptomic methods hold immense promise for spatially resolved mapping of cell types across human tissues. Here, we describe a protocol for automated single-molecule fluorescent in situ hybridization (smFISH) on standard histology sections at… read more here.
Keywords: fluorescent situ; single molecule; molecule fluorescent; rnascope ... See more keywords
Palmitoylation-Dependent Small-Molecule Fluorescent Probes for Live-Cell Golgi Imaging.
Sign Up to like & getrecommendations! Published in 2023 at "ACS chemical biology"
DOI: 10.1021/acschembio.3c00046
Abstract: Small-molecule fluorescent probes enabling visualization of the Golgi apparatus in living cells are essential tools for studying Golgi-associated biological processes and diseases. So far, several fluorescent Golgi stains have been developed by linking ceramide lipids… read more here.
Keywords: small molecule; cell; live cell; golgi ... See more keywords
Ultrasensitive Small-Molecule Fluorescent Thermometer Reveals Hot Mitochondria in Surgically Resected Human Tumors.
Sign Up to like & getrecommendations! Published in 2022 at "ACS sensors"
DOI: 10.1021/acssensors.2c01563
Abstract: The Warburg effect suggests that upregulated glycolysis arising from high glucose uptake in cancer cells might be accompanied with suppressed mitochondrial respiration. However, recent studies have shown that the mitochondrial temperature in cancer cells could… read more here.
Keywords: small molecule; cancer; mitochondrial temperature; hot mitochondria ... See more keywords
Recent advances in small-molecule fluorescent probes for studying ferroptosis.
Sign Up to like & getrecommendations! Published in 2022 at "Chemical Society reviews"
DOI: 10.1039/d1cs01167g
Abstract: Ferroptosis is an iron-dependent, non-apoptotic form of programmed cell death driven by excessive lipid peroxidation (LPO). Mounting evidence suggests that the unique modality of cell death is involved in the development and progression of several… read more here.
Keywords: small molecule; studying ferroptosis; fluorescent probes; ferroptosis ... See more keywords
dotdotdot: an automated approach to quantify multiplex single molecule fluorescent in situ hybridization (smFISH) images in complex tissues
Sign Up to like & getrecommendations! Published in 2020 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkaa312
Abstract: Abstract Multiplex single-molecule fluorescent in situ hybridization (smFISH) is a powerful method for validating RNA sequencing and emerging spatial transcriptomic data, but quantification remains a computational challenge. We present a framework for generating and analyzing… read more here.
Keywords: fluorescent situ; single molecule; multiplex single; complex tissues ... See more keywords
Ultrastructural visualization of chromatin in cancer pathogenesis using a simple small-molecule fluorescent probe
Sign Up to like & getrecommendations! Published in 2022 at "Science Advances"
DOI: 10.1126/sciadv.abm8293
Abstract: Imaging chromatin organization at the molecular-scale resolution remains an important endeavor in basic and translational research. Stochastic optical reconstruction microscopy (STORM) is a powerful superresolution imaging technique to visualize nanoscale molecular organization down to the… read more here.
Keywords: cancer; small molecule; chromatin; fluorescent probe ... See more keywords
Development of Small-Molecule Fluorescent Probes Targeting Enzymes
Sign Up to like & getrecommendations! Published in 2022 at "Molecules"
DOI: 10.3390/molecules27144501
Abstract: As biological catalysts, enzymes are vital in controlling numerous metabolic reactions. The regulation of enzymes in living cells and the amount present are indicators of the metabolic status of cell, whether in normal condition or… read more here.
Keywords: small molecule; fluorescent; development small; fluorescent probes ... See more keywords