Direct Measurements of the Cobalt‐Thiolate Bonds Strength in Rubredoxin by Single‐Molecule Force Spectroscopy
Sign Up to like & getrecommendations! Published in 2022 at "ChemBioChem"
DOI: 10.1002/cbic.202200165
Abstract: Cobalt is a trace transition metal. Although it is not abundant on earth, tens of cobalt‐containing proteins exist in life. Moreover, the characteristic spectrum of Co(II) ion makes it a powerful probe for the characterization… read more here.
Keywords: single molecule; spectroscopy; force spectroscopy; force ... See more keywords
Single‐Molecule Force Spectroscopy Reveals Stability of mitoNEET and its [2Fe2Se] Cluster in Weakly Acidic and Basic Solutions
Sign Up to like & getrecommendations! Published in 2022 at "ChemistryOpen"
DOI: 10.1002/open.202200056
Abstract: Abstract The outer mitochondrial membrane protein mitoNEET (mNT) is a recently identified iron‐sulfur protein containing a unique Fe2S2(His)1(Cys)3 metal cluster with a single Fe−N(His87) coordinating bond. This labile Fe−N bond led to multiple unfolding/rupture pathways… read more here.
Keywords: single molecule; spectroscopy; cluster; molecule force ... See more keywords
Improving small molecule force fields by identifying and characterizing small molecules with inconsistent parameters
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Computer-Aided Molecular Design"
DOI: 10.1007/s10822-020-00367-1
Abstract: Many molecular simulation methods use force fields to help model and simulate molecules and their behavior in various environments. Force fields are sets of functions and parameters used to calculate the potential energy of a… read more here.
Keywords: small molecule; force; force fields; molecule force ... See more keywords
Complex Dynamics in Single Molecule Force Spectroscopy from Simple Simulation Models
Sign Up to like & getrecommendations! Published in 2017 at "Biophysical Journal"
DOI: 10.1016/j.bpj.2016.11.1085
Abstract: Single molecule force spectroscopy studies of protein folding dynamics have yielded unprecedented insight into the mechanisms of the folding reaction. Still, they usually allow for a very limited description of the process of interest (e.g.… read more here.
Keywords: single molecule; force spectroscopy; force; spectroscopy ... See more keywords
Harnessing Entropy in Single-Molecule Force Spectroscopy with Semiconducting Polymers
Sign Up to like & getrecommendations! Published in 2018 at "Chem"
DOI: 10.1016/j.chempr.2018.01.014
Abstract: In this issue of Chem , Sprakel and co-workers use a fluorescence-doped flexible semiconducting polymer to construct a single-molecule sensor that can detect ultra-weak forces in the molecular environment with a grayscale down to 300… read more here.
Keywords: single molecule; molecule force; spectroscopy; harnessing entropy ... See more keywords
Single-Molecule Force Spectroscopy Reveals Adhesion-by-Demand in Statherin at the Protein–Hydroxyapatite Interface
Sign Up to like & getrecommendations! Published in 2020 at "Langmuir"
DOI: 10.1021/acs.langmuir.0c02325
Abstract: Achieving strong adhesion in wet environments remains a technological challenge in biomedical applications demanding biocompatibility. Attention for adhesive motifs meeting such demands has largely been focused on marine organisms. However, bioadhesion to inorganic surfaces is… read more here.
Keywords: single molecule; force spectroscopy; adhesion; spectroscopy ... See more keywords
Two-Molecule Force Spectroscopy on Proteins.
Sign Up to like & getrecommendations! Published in 2023 at "ACS nano"
DOI: 10.1021/acsnano.3c01185
Abstract: Many elastomeric proteins, which play important roles in a wide range of biological processes, exist as parallel/antiparallelly arranged dimers or multimers to perform their mechanobiological functions. For example, in striated muscle sarcomeres, the giant muscle… read more here.
Keywords: two molecule; spectroscopy; elastomeric proteins; force spectroscopy ... See more keywords
Elastin-like Polypeptide Linkers for Single-Molecule Force Spectroscopy.
Sign Up to like & getrecommendations! Published in 2017 at "ACS nano"
DOI: 10.1021/acsnano.7b02694
Abstract: Single-molecule force spectroscopy (SMFS) is by now well established as a standard technique in biophysics and mechanobiology. In recent years, the technique has benefitted greatly from new approaches to bioconjugation of proteins to surfaces. Indeed,… read more here.
Keywords: single molecule; force spectroscopy; elastin like; force ... See more keywords
SpyTag/SpyCatcher tether as a fingerprint and force marker in single-molecule force spectroscopy experiments.
Sign Up to like & getrecommendations! Published in 2021 at "Nanoscale"
DOI: 10.1039/d1nr01907d
Abstract: Single molecule force spectroscopy has emerged as a powerful tool to study protein folding dynamics, ligand-receptor interactions, and various mechanobiological processes. High force precision does not necessarily lead to high force accuracy, as the uncertainties… read more here.
Keywords: single molecule; force spectroscopy; force; spectroscopy ... See more keywords
Quantifying the Native Energetics Stabilizing Bacteriorhodopsin by Single-Molecule Force Spectroscopy.
Sign Up to like & getrecommendations! Published in 2020 at "Physical review letters"
DOI: 10.1103/physrevlett.125.068102
Abstract: We quantified the equilibrium (un)folding free energy ΔG_{0} of an eight-amino-acid region starting from the fully folded state of the model membrane-protein bacteriorhodopsin using single-molecule force spectroscopy. Analysis of equilibrium and nonequilibrium data yielded consistent,… read more here.
Keywords: single molecule; force spectroscopy; molecule force; spectroscopy ... See more keywords