Analyzing Single Molecule FRET Trajectories Using HMM.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6753-7_7
Abstract: Structural dynamics of biomolecules, such as proteins, plays essential roles in many biological phenomena at molecular level. It is crucial to understand such dynamics in recent biology. The single-molecule Förster resonance energy transfer (smFRET) measurement… read more here.
Keywords: hmm; single molecule; molecule fret; biology ... See more keywords
Single-Molecule FRET Assay to Observe the Activity of Proteins Involved in RNA/RNA Annealing.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7634-8_17
Abstract: In recent years, single-molecule fluorescence resonance energy transfer (smFRET) has emerged as a powerful technique to study macromolecular interactions. The chief advantages of smFRET analysis compared to bulk measurements include the possibility to detect sample… read more here.
Keywords: methodology; single molecule; rna; molecule fret ... See more keywords
Single-Molecule FRET Analysis of Replicative Helicases.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8556-2_12
Abstract: Over the recent years single-molecule fluorescence resonance energy transfer (smFRET) technique has proven to be one of the most powerful tools for revealing mechanistic insights into helicase activities. Here we describe details of single-molecule FRET… read more here.
Keywords: replicative helicases; molecule; single molecule; molecule fret ... See more keywords
Single-molecule FRET studies on interactions between elongation factor 4 (LepA) and ribosomes
Sign Up to like & getrecommendations! Published in 2017 at "Chinese Chemical Letters"
DOI: 10.1016/j.cclet.2017.12.006
Abstract: Abstract Elongation factor 4 (EF4) is one of the highly conserved translational GTPases, whose functions are largely unknown. Structures of EF4 bound ribosomal PRE-translocation and POST-translocation complexes have both been visualized. On top of cellular,… read more here.
Keywords: molecule fret; translocation; single molecule; elongation factor ... See more keywords
Single-molecule FRET reveals multiscale chromatin dynamics modulated by HP1α
Sign Up to like & getrecommendations! Published in 2017 at "Nature Communications"
DOI: 10.1038/s41467-017-02619-5
Abstract: The dynamic architecture of chromatin fibers, a key determinant of genome regulation, is poorly understood. Here, we employ multimodal single-molecule Förster resonance energy transfer studies to reveal structural states and their interconversion kinetics in chromatin… read more here.
Keywords: single molecule; chromatin; chromatin fibers; fret reveals ... See more keywords
Precision and accuracy of single-molecule FRET measurements—a multi-laboratory benchmark study
Sign Up to like & getrecommendations! Published in 2018 at "Nature Methods"
DOI: 10.1038/s41592-018-0085-0
Abstract: Single-molecule Förster resonance energy transfer (smFRET) is increasingly being used to determine distances, structures, and dynamics of biomolecules in vitro and in vivo. However, generalized protocols and FRET standards to ensure the reproducibility and accuracy… read more here.
Keywords: fret efficiencies; molecule; multi laboratory; single molecule ... See more keywords
Dynamics of the nucleosomal histone H3 N-terminal tail revealed by high precision single-molecule FRET
Sign Up to like & getrecommendations! Published in 2020 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkz1186
Abstract: Abstract Chromatin compaction and gene accessibility are orchestrated by assembly and disassembly of nucleosomes. Although the disassembly process was widely studied, little is known about the structure and dynamics of the disordered histone tails, which… read more here.
Keywords: terminal tail; single molecule; dna; histone terminal ... See more keywords
Single-Molecule FRET: A Tool to Characterize DNA Nanostructures
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Molecular Biosciences"
DOI: 10.3389/fmolb.2022.835617
Abstract: DNA nanostructures often involve temporally evolving spatial features. Tracking these temporal behaviors in real time requires sophisticated experimental methods with sufficiently high spatial and temporal resolution. Among the several strategies developed for this purpose, single-molecule… read more here.
Keywords: molecule fret; single molecule; dna; fret tool ... See more keywords