Workflows of the Single-Molecule Imaging Analysis in Living Cells: Tutorial Guidance to the Measurement of the Drug Effects on a GPCR.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1258-3_32
Abstract: Single-molecule imaging (SMI) is a powerful method to measure the dynamics of membrane proteins on the cell membrane. The single-molecule tracking (SMT) analysis provides information about the diffusion dynamics, the oligomer size distribution, and the… read more here.
Keywords: molecule; single molecule; analysis; molecule imaging ... See more keywords
Single-Molecule Imaging of Escherichia coli Transmembrane Proteins.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7271-5_7
Abstract: Single-molecule imaging in living cells can provide unique information about biological processes. Bacteria offer some particular challenges for single-molecule imaging due to their small size, only slightly larger than the diffraction limit of visible light.… read more here.
Keywords: molecule; single molecule; escherichia coli; imaging escherichia ... See more keywords
Unraveling of Lipid Raft Organization in Cell Plasma Membranes by Single-Molecule Imaging of Ganglioside Probes.
Sign Up to like & getrecommendations! Published in 2018 at "Advances in experimental medicine and biology"
DOI: 10.1007/978-981-13-2158-0_3
Abstract: Ganglioside s are involved in a variety of physiological roles and particularly in the formation and function of lipid rafts in cell membranes. However, the dynamic behaviors of gangliosides have not been investigated in living… read more here.
Keywords: single molecule; raft; ganglioside probes; molecule imaging ... See more keywords
Single-Molecule Imaging of Proteoglycans in the Pericellular Matrix.
Sign Up to like & getrecommendations! Published in 2017 at "Biophysical journal"
DOI: 10.1016/j.bpj.2017.09.030
Abstract: The pericellular matrix is a robust, hyaluronan-rich polymer brush-like structure that controls access to the cell surface, and plays an important role in cell adhesion, migration, and proliferation. We report the observation of single bottlebrush… read more here.
Keywords: single molecule; pericellular matrix; matrix; molecule imaging ... See more keywords
In Situ Single-Molecule Imaging of MicroRNAs in Switchable Migrating Cells under Biomimetic Confinement.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.1c05223
Abstract: Spatial imaging of RNAs in single cells is extremely charming for deciphering of regulatory mechanisms in multiple migration modes during tumor metastasis. Herein, enzyme-free-mediated cascade amplified nanoprobes were designed for in situ single-molecule imaging of… read more here.
Keywords: migrating cells; situ single; single molecule; switchable migrating ... See more keywords
Zinc Finger-Mediated Labeling Reveals the Stoichiometry of Membrane Proteins.
Sign Up to like & getrecommendations! Published in 2020 at "ACS nano"
DOI: 10.1021/acsnano.9b08865
Abstract: Characterizing interactions of proteins is pivotal for understanding their function. Recently, single- molecule imaging based methods have proven useful for directly testing the stoichiometry of multi-subunit protein complexes. A limiting factor is the labeling of… read more here.
Keywords: protein; single molecule; zinc finger; molecule imaging ... See more keywords
Revisiting Kekulene: Synthesis and Single-Molecule Imaging
Sign Up to like & getrecommendations! Published in 2019 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.9b07926
Abstract: Four decades after the first (and only) reported synthesis of kekulene, this emblematic cycloarene has been obtained again through an improved route involving the construction of a key synthetic intermediate, 5,6,8,9-tetrahydrobenzo[m]tetraphene, by means of a… read more here.
Keywords: single molecule; synthesis single; revisiting kekulene; molecule imaging ... See more keywords
Ultrafast isolated molecule imaging without crystallization
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2122793119
Abstract: Significance Excitation of molecules by an ultrashort laser pulse creates rotational wave packets that lead to transient alignment of the molecules along the laser polarization direction. Here, we show that a train of ultrashort laser… read more here.
Keywords: molecule imaging; laser; imaging without; isolated molecule ... See more keywords
Single-molecule imaging and tracking of molecular dynamics in living cells
Sign Up to like & getrecommendations! Published in 2017 at "National Science Review"
DOI: 10.1093/nsr/nww055
Abstract: Unlike the ensemble-averaging measurements, the single-molecule imaging and tracking (SMIT) in living cells provides the real-time quantitative information about the locations, kinetics, dynamics and interactions of individual molecules in their native environments with high spatiotemporal… read more here.
Keywords: molecule; single molecule; tracking molecular; molecule imaging ... See more keywords
Protein detection in blood with single-molecule imaging
Sign Up to like & getrecommendations! Published in 2021 at "Science Advances"
DOI: 10.1126/sciadv.abg6522
Abstract: A single-molecule imaging approach detects individual disease-specific protein molecules in blood samples. The ability to characterize individual biomarker protein molecules in patient blood samples could enable diagnosis of diseases at an earlier stage, when treatment… read more here.
Keywords: molecule; detection; single molecule; molecule imaging ... See more keywords
Combined modelling of mRNA decay dynamics and single-molecule imaging in the Drosophila embryo uncovers a role for P-bodies in 5′ to 3′ degradation
Sign Up to like & getrecommendations! Published in 2023 at "PLOS Biology"
DOI: 10.1371/journal.pbio.3001956
Abstract: Regulation of mRNA degradation is critical for a diverse array of cellular processes and developmental cell fate decisions. Many methods for determining mRNA half-lives rely on transcriptional inhibition or metabolic labelling. Here, we use a… read more here.
Keywords: single molecule; molecule imaging; drosophila embryo; mrna ... See more keywords